International Journal for Multidisciplinary Research (IJFMR) E-ISSN: 2582-2160 ● Website: www.ijfmr.com ● Email: editor@ijfmr.com IJFMR22061076 Volume 4, Issue 6, November-December 2022 1 Ethnomedicinal Survey and a Quantitative Analysis of Bioactive Plants Used by Sonowal Kachari Community of Assam Himadri Saikia 1 , Nabanita Maity 2 , Mahuya Sengupta 3* 1,2,3 Department of Biotechnology, Assam University, Silchar, Assam, India- 788011 *Corresponding author: senguptamahuya35@gmail.com Abstract Background In recent times, cases of infectious and malignant diseases are increasing in an exponential manner. The treatments to these diseases face drawbacks of specificity and high costs. Due to this, many patients seek alternative or complementary methods of treatment. Ethnomedicine is the first and foremost choice in this regard. However due to cultural belief no proper documentation with scientific validation is available. The present study intended to validate the potentiality of the ethnomedicinal plants of Sonowal Kachari community of Assam, quantitatively and qualitatively along with a few bioactivity studies of oxidative stress and antioxidant defences in a murine immune model. Methods The study was based on extensive ethnomedicinal field survey for 1 year period from 2015-2016. Data was analysed and most potent plant was short listed using disease consensus index (DCI), phytochemical screening and 2,2-diphenylpicrylhydrazyl (DPPH) assay. An effective dose was determined by calculating IC50 with 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction (MTT) assay in murine splenic macrophages. Bioactivity were studied in lipopolysaccharide (LPS) stimulated murine splenic macrophages for oxidative stress and antioxidant parameters. Results From the preliminary quantitative and qualitative screening it was found that Oroxylum indicum methanol extract (OIM) has a potent antioxidant activity. Based on IC50 (inhibitory concentration for 50% viability) value a working dose of 4.76 μg/ml was determined. The oxidative stress and antioxidant parameters show high free radical scavenging activity of OIM. Conclusion Systemic pharmacological trials in present study validate the active bioactive potential of the ethnomedicinal plant as per scientific standards. Keywords: Ethnomedicine, Oxidative stress, Antioxidant, Sonowal Kachari.