Int.J.Curr.Microbiol.App.Sci (2022) 11(09): 244-251 244 Original Research Article https://doi.org/10.20546/ijcmas.2022.1109.027 Induced Expression of Ovarian Heat Shock Proteins with Response to Thermal Stress in an Endemic Freshwater Crab, Sartoriana spinigera (Wood-mason, 1871) from East Coast of India Pradipta Pati 1 , Debasmita Dubey 2 and Gopal Krishna Purohit 3* 1 Christ College, Kanika Road, Cuttack, Odisha – 753008, India 2 Siksha O Anusandhan University, Bhubaneswar, Odisha – 751003, India 3 Heredity Biosciences LLP. Plot No-818, Mayfair lagoon road, Jayadev Vihar, Bhubaneswar, Odisha – 751015, India *Corresponding author ABSTRACT Introduction The most difficult environmental issue influencing the reproductive physiology of various species, including mammals, fish, and birds, is the rise in ambient temperature brought on by global warming. In order to mitigate this negative consequence, organisms create a variety of different survival methods. In this regard, the heat shock response (HSR) is a highly conserved cellular process International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 11 Number 09 (2022) Journal homepage: http://www.ijcmas.com The Heat shock proteins (HSP) play an important role in controlling the apoptosis and inflammatory response. These proteins are released into the extracellular environment and act as mediators of inflammation. Protein folding accuracy is ensured by chaperone activity, which also prevents the accumulation of abnormal isoforms. In the current work, Sartoriana spinigera, an endemic freshwater crab, is examined for its survival and Hsps expression patterns in ovarian tissues in response to short-term heat stress. Crabs maintained at 25 o C served as control. Expression of Hsp genes were analyzed using semi-quantitative and quantitative PCR in both groups to see whether these crabs have lost the Hsp gene plasticity. Hsp gene expression analysis showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in heat stressed crabs, whereas there was no alteration in expression of other Hsps. Up-regulation of all the Hsps was observed in the heat shocked crabs, whereas expression of all Hsps were down regulated to the basal level in crabs maintained at 34 o C/24 hour. These results indicate that Hsp90 and Hsp60 play important role in survival of Sartoriana spinigera under thermal stress. Keywords Sartoriana spinigera, Heat stress, Thermal acclimation, Hsps, RT-qPCR Received: 05 August 2022 Accepted: 29 August 2022 Available Online: 10 September 2022 Article Info