S1 Design and synthesis of fluorescence-labeled nucleotide with cleavable azo linker for DNA sequencing Lianjiang Tan † , Yazhi Liu †,‖ , Qinglai Yang † , Xin-Yan Wu ‖ , Xiaowei Li § , Bing Gong ¶,‡ Yu-Mei Shen †,* and Zhifeng Shao § † Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Collaborative Innovation Center of Systems Biomedicine, and § Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China ‖ Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, China ¶ College of Chemistry, Beijing Normal University, Beijing 100875, China ‡ Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260, USA Supporting Information Contents General Methods S2 Experimental Section S2-S5 Supplementary Data S6-S7 DNA Extension/Cleavage Cycles S7-S11 Copies of 1 NMR, 13 C NMR and 31 P NMR spectra S12-S20 Electronic Supplementary Material (ESI) for ChemComm. This journal is © The Royal Society of Chemistry 2015