S1 Persistent Organic Nanopores Amenable to Structural and Functional Tuning Xiaoxi Wei, †¶‡ Guoqing Zhang, †‡ Yi Shen, ║‡ Yulong Zhong, † Rui Liu, †¶ Na Yang, † Fayez Y. Al-mkhaizim, ¶ Mark Kline, ¶ Lan He, § Minfeng Li, † Zhong-Lin Lu, †* Zhifeng Shao, ║* and Bing Gong ¶†* † College of Chemistry, Beijing Normal University, Beijing 100875, China ¶ Department of Chemistry, The State University of New York at Buffalo, Buffalo, New York 14260, United States ║ School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China § National Institute for Food and Drug Control, Beijing 100050, China Supporting Information I. General Information S2 II. Synthesis and Characterization of Macrocycles 2 S3 III. 1 H and 13 C NMR Spectra of Macrocycles 2 S6 IV. High-Resolution MALDI-FT-ICR Spectra of Macrocycles 2 S10 V. Synthesis and Characterization of Precursors S14 VI. 1 H and 13 C NMR Spectra of Precursors S24 VII. 1 H NMR Comparison of 2 in Different Solvents S29 VIII. Fluorescence Spectra S37 IX. CD spectra S39 X. Atomic Force Microscopy S40 XI. Stopped-Flow Kinetic Assays S44 XII. Liposome-Based Assays on Chloride Transport S45 XIII. Single Channel Electrophysiology S46