_____________________________________________________________________________________________________ *Corresponding author: Email: von73vv22@yahoo.com; Int. J. Trop. Dis. Health, vol. 45, no. 4, pp. 36-45, 2024 International Journal of TROPICAL DISEASE & Health Volume 45, Issue 4, Page 36-45, 2024; Article no.IJTDH.114121 ISSN: 2278–1005, NLM ID: 101632866 Prevalence and Concentration of Airborne Microorganisms Isolated from Domestic Toilets in Port Harcourt, Rivers State, Nigeria Agi V. N. a* , Ollor O. A. a , Azike C. A. a and Chukwu G. C. a a Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJTDH/2024/v45i41526 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/114121 Received: 02/01/2024 Accepted: 07/03/2024 Published: 07/03/2024 ABSTRACT Introduction: Microorganisms are ubiquitous in nature and transient airborne microorganisms have been shown to constitute major health hazards. Aim: This study was carried out to assess the microbial air quality in ten (10) domestic toilets located in mile-3, Alakahia, Choba, Ada-George and Rumuokwuta, Port Harcourt, Rivers state, Nigeria. Methodology: Microbial air quality was sampled using sedimentation techniques. Freshly prepared Nutrient Agar, MacConkey and Sabouraud Dextrose Agar (SDA) plates were placed one meter above the floor of the toilets for 1 hours at different sections of each toilet exposed to an open air. The agar plates were closed and transported to the Laboratory were incubation took place at 37°C for 24 hours and 25°C for 3-5 days for growth of bacteria and fungi respectively. Isolates were characterized and identified by standard microbiological methods. Results: The bacteria isolated were Staphylococcus species 22(22.45%), Bacillus species 20 Original Research Article