Int.J.Curr.Microbiol.App.Sci (2020) 9(12): 137-143 137 Original Research Article https://doi.org/10.20546/ijcmas.2020.912.018 Genetic Diversity Analysis of Indian Mustard (Brassica spp.) Germplasm Lines using SSR Molecular Markers Ravi Baghel 1 , A. K. Sharma 1 , Sushma Tiwari 1 , M. K. Tripathi 1* and Niraj Tripathi 2 1 Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior-474002, India 2 Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur-482004, India *Corresponding author ABSTRACT Introduction Oilseed Brassica occupies an imperative spot in the agriculture due to useful oil content. In most of the provinces of the world, its cultivation has risen tremendously during the last periods and, by now; it is the third largest contributor of the world supply of vegetable oil. Rapeseed is one of the most significant oil and protein rich annual crops in the world. Seed provides oil both for industrial and cooking purposes. The oils extracted from mustard contain high protein (37%) and feed concentration which is highly palatable to livestock. So, it is urgent to analyse the genetic diversity and its response for the selection of short duration mustard genotypes for increasing our cropping intensity. Presence of diversity at marker loci is presently the most feasible strategy for characterizing diversity among mustard germplasm lines. Molecular markers offer the best estimate of genetic diversity, since these are independent from the difficult effects of environmental factors. In mustard, various International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 12 (2020) Journal homepage: http://www.ijcmas.com Molecular categorization of 48 mustard (Brassica spp.) genotypes by employing 20 SSR markers divulged that eight primers viz., BRMS-240, BRMS-324, SR-7223, SR- 9222, SR-94102, OI10-C05, SSRNa10-DO9 and SSRNa 10-D11 illustrated good technical resolution and out of these eight seven furnished adequate variations among diverse genotypes. In total 50% polymorphism was detected. Major group restrained 48 genotypes that divided into three major groups, first main group ‘I’ contained 17 genotypes, second chief group ‘II’ hold 24 genotypes and third core group ‘III’ included 7 genotypes. Polymorphic information content (PIC) was estimated for each of the 7 SSR markers. The highest PIC value (0.6851) was documented for the molecular marker OI10-CO 5. It had 8 alleles among the 48 genotypes; whereas, the lowest PIC (0.4038) was examined for the molecular marker SR-7223. Keywords Indian mustard, Cluster analysis, Diversity analysis, SSR marker Accepted: 04 November 2020 Available Online: 10 December 2020 Article Info