Revathi Chitra Santhanam/Afr.J.Bio.Sc.6.12(2024) ISSN: 2663-2187 https://doi.org/10.48047/AFJBS.6.12.2024.1763-1784 Isolation of Fucoidan from seaweed Turbinaria conoides and evaluation of its anticancer potential on animal models Revathi Chitra Santhanam 1 , Syed Ali Mohamed Yacoob 2 *, Dr. R. Rathinam 3 , DR. Sirisha Chittala 4 , Kavitha M.B 5 , P Radha 6 , Ruban P 7 , Anuradha Venkatraman 8 , Dr. N. Yogananth Nagaraja 9 1 Department of Biotechnology, New Prince Shri Bhavani Arts & Science College, Affiliated to the University of Madras, Chennai, TamilNadu, India. 2 Department of Biotechnology, Mohamed Sathak College of Arts and Science, Affiliated to the University of Madras, Chennai, TamilNadu, India. 3 Department of Chemistry, Sri Eshwar College of Engineering, Affiliated to Anna University, Coimbatore, Tamilnadu, India. 5 Department of Microbiology, Shri Nehru Maha Vidyala College of arts and science, Coimbatore-50, India 6 Department of Microbiology, Shri Nehru Maha Vidyala College of arts and science, Coimbatore-50, India 7 Department of Biotechnology & Research, Shri Nehru Maha Vidyala College of arts and science, Coimbatore-50, India 8 Department of Biochemistry, Mohamed Sathak College of Arts and Science, Affiliated to the University of Madras, Chennai, TamilNadu, India. 9 PG and Research Department of Microbiology, Mohamed Sathak College of Arts and Science, Affiliated to the University of Madras, Chennai, TamilNadu, India. Corresponding Author: Syed Ali Mohammed Yahoob, email:syedmicro555@gmail.com Article History Volume 6 Issue 12, 2024 Received: 25 May 2024 Accepted : 30 June 2024 doi: 10.48047/AFJBS.6.12.2024.1763-1784 Abstract Background: In this study, Fucoidan a type of polysaccharide extracted from Turbinaria conoides was subjected to invivo acute toxicity, sub-acute toxicity study, and invivo anticancer study to find fucoidan effect on Sprague Dawley rat (SD rats). An acute toxicity test was executed on matured Sprague Dawley rats. Result:They were administered with a single dose of 500mg/kg of fucoidan extract for 14 consecutive days and their general behavior, mental and physical changes along with mortality were observed and then the SD rats were subjected to the Sub-acute test and they were administrated with 50mg/kg, 100mg/kg, and 200mg/kg of fucoidan through oral gavage along with Tween 20 for 28 consecutive days and on the 29 day the animals were sacrificed and their vital organs were dissected and analyzed for noticeable signs of toxicity. Thus the study was extended to invivo anticancer analysis based on the results obtained from the acute and sub- acute toxicity studies to find the breast anticancer potential of the fucoidan. 250 mg/ml of test sample fucoidan was administered to 25mg/kg DMBA induced group animals (breast