Yadav et al., IJPSR, 2025; Vol. 16(3): 727-732. E-ISSN: 0975-8232; P-ISSN: 2320-5148 International Journal of Pharmaceutical Sciences and Research 727 IJPSR (2025), Volume 16, Issue 3 (Research Article) Received on 25 October 2024; received in revised form, 30 December 2024; accepted, 24 February 2025; published 01 March 2025 SYNTHESIS OF NOVEL SPIROTHIAZOLIDINONE DERIVATIVES AND EVALUATION OF ANTI-INFLAMMATORY ACTION Ashish Kumar Yadav, Muraree Lal, Munesh Singh Bhadauria, Narottam Singh and Avinash K. Kondalkar * Sun Institute of Pharmaceutical Education and Research, Lahar - 477445, Madhya Pradesh, India. ABSTRACT: The objective of this investigation was to synthesize spirothiazolidinone-chalcone derivatives and evaluate them for anti- inflammatory action. Five 4-substituted benzaldehydes were utilized for the aldol condensation leading to chalcones of spirothiazolidinone. The compounds were obtained in yield of 64-72% and displayed varying solubility with all compounds soluble in chloroform and DMSO. The NMR spectra revealed protons of amine, imine, hydroxy and aromatic groups. The presence of the molecular ion peak of the isotopic peak was found in the mass spectra of the compounds confirming the formation of the compounds. The anti-inflammatory activity was determined using the albumin denaturation method and antiprotease method. All the compounds exhibited dose dependent inhibition of albumin denaturation with 7d having the highest capacity to cause the inhibition (61.56 ± 1.033 %) at the concentration of 500μg/mL. The antiprotease action was also dose dependent and 7d at 500μg/mL was able to inhibit (46.32±3.011 %) of protease activity. The type of substitution on the chalcone phenyl ring played a vital role in the activity of the compounds. The results led to the conclusion that newer chalcone based molecules with anti-inflammatory activity were obtained from the current work. INTRODUCTION: Spirocyclic compounds isolated from plant and animal origins have important applications in medicinal chemistry 1 . Spiro compounds having cyclic structures fused at a central carbon are of recent interest because of their interesting conformational features and their structural implications for biological systems. The asymmetric characteristic of the molecule due to the chiral spiro carbon is one of the important criteria of biological activities. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.16(3).727-32 This article can be accessed online on www.ijpsr.com DOI link: https://doi.org/10.13040/IJPSR.0975-8232.16(3).727-32 Spiro compounds represent an important class of naturally occurring substances characterized by their highly pronounced biological properties 2-6 . Although, a number of molecules have been developed as antimicrobial agents. But the demand for the effective and potent antimicrobial agent is always on high priority due to the development of resistant for the current drugs. Spiro moiety is part of various natural products and medicinal agents. In literature, it has been reported that sharing of the indole-3 carbon atom during formation of spirothiazolidinone derivatives greatly enhances its biological activity. Thus, considering this fact, we planned to synthesize some spirothiazolidinone derivatives to improve antimicrobial efficacy. Keywords: Anti-inflammatory, Chalcone, Spirothiazolidinone, Albumin Denaturation, Protease Correspondence to Author: Avinash K. Kondalkar Sun Institute of Pharmaceutical Education and Research, Lahar - 477445, Madhya Pradesh, India. E-mail: siperpg@gmail.com