International Journal of Advances in Applied Sciences (IJAAS) Vol. 14, No. 1, March 2025, pp. 175~184 ISSN: 2252-8814, DOI: 10.11591/ijaas.v14.i1.pp175-184  175 Journal homepage: http://ijaas.iaescore.com Identification and antioxidant activity test of β-tocopherol from Dompu corn oil as anti-aging Adi Permadi 1 , Pramudita Budiastuti 2 , Barry Nur Setyanto 2 , Rachmadian Wulandana 3 , Fatma Nuraisyah 4 , Putri Rachma Novitasari 5 , Arief Syamsuddin 6 , Muhammad Kaisar Sutomo Ramadhan 7 , Andi Rifki Rosandy 8 1 Department of Chemical Engineering, Faculty of Industrial Technology, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 2 Department of Electronic Technology Vocational Education, Faculty of Teacher Training and Education, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 3 Mechanical Engineering Program, State University of New York (SUNY), New Paltz, United States 4 Department of Public Health, Faculty of Public Health, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 5 Department of Pharmacy, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 6 Department of Automotive Technology Vocational Education, Faculty of Teacher Training and Education, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 7 Department of Chemical Engineering, Faculty of Industrial Technology, Universitas Ahmad Dahlan, Yogyakarta, Indonesia 8 EBM SciTech, Research Center for Bioscience and Biotechnology Institut Teknologi Bandung, Bandung, Indonesia Article Info ABSTRACT Article history: Received Feb 10, 2024 Revised Oct 29, 2024 Accepted Dec 26, 2024 Corn is a well-cultivated food crop that is grown extensively worldwide. The used portion of corn is its seeds, which are rich in oil. The objective of this study is to separate and characterize tocopherol from corn oil in Dompu, Sumbawa by employing spectroscopic techniques such as ultraviolet (UV) spectrophotometry, ultra-performance liquid chromatography (UPLC), nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Subsequently, the in-vitro antioxidant activity of the tocopherol was assessed. 1 kilogram of dry maize kernels subjected to 70% ethanol extraction yielded 30 grams (35 ml) of corn oil. The purified isolate obtained from the fractionated extract, using radial chromatography, demonstrated the presence of tocopherol. The isolated sample exhibited the presence of beta-tocopherol. Beta-tocopherol's anti-aging properties were assessed by conducting an in-vitro antioxidant activity test utilizing the tyrosinase enzyme. The IC50 value obtained was 83.954±2.849 ppm. The IC50 value indicates that beta-tocopherol possesses significant antioxidant activity, making it suitable for usage as a primary ingredient in cosmetics and pharmaceutical products. Keywords: Anti-aging Corn oil Tyrosinase enzyme Vitamin E β-tocopherol This is an open access article under the CC BY-SA license. Corresponding Author: Adi Permadi Department of Chemical Engineering, Faculty of Industrial Technology, Universitas Ahmad Dahlan Jl. Kapas 9, Semaki, Umbulharjo, Yogyakarta 55166, Indonesia Email: adi.permadi@che.uad.ac.id 1. INTRODUCTION Corn is widely grown and is one of the world's largest food crops. Corn is a commonly consumed food crop in Indonesia and serves as a prominent item in the agricultural sector of Dompu, Sumbawa, West Nusa Tenggara. According to relevant research, in developed countries, corn is mainly used for animal feed and human consumption. The oil content of the kernel is primarily determined by the percentage of oil present in the germ and the proportion of the germ within the caryopsis. The majority of commercially available maize oil is derived from the germ, which is frequently referred to as "corn germ oil" [1]. Vegetable