POSTER PRESENTATIONS 39 Second Day Iranian Journal of Kidney Diseases | Volume 3 | Supplement 1 | December 2009 P201 Effect of Grape Seed Extract on Apoptosis in Experimental Diabetic Nephropathy Doustar Y, Sadig Etehad S, Gavami S Islamic Azad University, Tabriz Branch, Veterinary Medicine College, Veterinary Pathology Department, Tabriz, IRAN Introduction. Diabetes mellitus and its complications are a public health problem. Antioxidant Materials are frequently recommended in the type І and type П diabetes mellitus and can decrease oxidative stress. The purpose of this study was to investigate the effect of grape seed extract on apoptosis in diabetic nephropathy. Methods. In this study, 56 twelve-week-old rats (200 to 300 gr) were selected and divided into two groups (case [treatment] and control).In these two groups, diabetes was induced by intraperitoneal injection of streptozocin (50 mg). The treatment groups were kept in normal conditions of food and place and treated by daily grape seed extract for 12 weeks. In control group, we had normal conditions (food and place).After 12 weeks, renal tissues were sampled in both groups and 5- to 6-micron tissue sections were prepared using H&E and TUNEL assay staining method. Results. Histopathological analysis of tissue sections in both groups demonstrated that the deference of histopathological changes and apoptosis were significant P < 0.005. Conclusion. We demonstrated that grape seed extract was able to reduce the oxidative stress and apoptotic changes. Also, grape seed extract improved diabetic nephropathy disease in patients resulting in the reduction of HbA1C, oxidative stress, hyperglycemia, VLDL, expression of apoptosis regulatory gene, and TGF- β, and increased insulin sensitivity, HSPG, HS, HDL, IGF, and EGF. P202 Quality of Life in Chronic Iranian Hemodialysis Patients Khavanin-zadeh M, Gholipour F Iran University of Medical Sciences, Tehran, IRAN Introduction. Quality of life (QoL) is an important predictor of outcome in End-Stage Renal Disease (ESRD) patients. Therefore, QoL needs to be regularly assessed in this setting. Our study describes QoL, as well as demographic and clinical variables associated with QoL in chronic Iranian haemodialysis (HD) patients. Methods. Prevalent chronic HD patients ( N = 970; mean age, 45.7 ± 12.6 years) in one dialysis centre from the twenty main regions of Iran were included in the study. All of them completed the Short-Form Health Survey (SF-36) and the Kidney Disease Quality of Life Questionnaire Short Form (KDQOL-SF). Results. Mean physical component summary (PCS) score was 64.3 ± 19.2 and the mean mental component summary (MCS) score was 45.1 ± 19.3.These figures were lower than those previously assessed in non-dialysis age-matched Iranian individuals. The mean kidney disease summary component (KDSC) score was 72.3 ± 11.3, similar to other studies. The worst dimension of QoL was work, whereas the best ones were cognitive function and quality of social interaction. We found older age, female gender, lower socio-economic status, and higher level to be associated with lower QoL scores. Conclusion. The QoL of HD patients in Iran is lower than that in the general population. Our results suggest that at least one-third of these patients may be considered for rehabilitation therapy, in order to try and prevent complications and mortality. P203 Incidence of Uric Acid Stones in Diabetics with Nephrolitiasis Aboutorabi R, Ahmadnia, Shakeri H, Rajabian R Mashhad University of Medical Sciences, Endocrinology and Metabolism Research, Mashhad, IRAN Introduction. Deficiency of amoniogenesis in diabetes results in decreased urinary PH and predisposition for renal stones. Increased incidence of uric acid stones has been reported in diabetic patients. We have studied the incidence of uric acid stones in diabetics with renal stones referring to Ghaem hospital in Mashhad during 2008-2009. Methods. From 210 (137 male and 73 female) patients between 30-70 year-old (mean age of 44.9±11.1) with renal stones. We studied 28 patients who had diabetes. Diabetes was considered if they had history of known diabetes or had FBS of ≥ to 126 mg/dl in 2 occasions. These patients did not have history of receiving corticosteroids, vitamin D, calcium supplements and diuretics. We measured FBS, BUN, Creatinine, Uric acid, Ca, P, alkaline phosphatase, urine analysis, 24 hour urinary uric acid. We studied PTH in patients who had abnormal Ca in 2 occasions. All removed stones were analyzed. Results. In diabetics the composition of stone was Calcium (50%), uric acid 46.4% and struvit 3.6%. In non diabetic patients composition of renal stone was calcium, 69.2%, uric acid 23.6% and struvit 7.1%. Conclusion. Diabetes may be a risk factor for Uric acid stones.