_____________________________________________________________________________________________________ *Corresponding author: E-mail: vanuradha372@gmail.com; Journal of Pharmaceutical Research International 33(31B): 203-211, 2021; Article no.JPRI.68948 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) A Newly Developed Reverse Phase-High Performance Liquid Chromatography Method for the Assay of Dexmethylphenidate and Serdexmethylphenidate with PDA Subrahmanyam Talari 1,2 , V. Anuradha 3* , S. N. Murthy Boddapati 2 and Komala Sai Prathuyasa Ayithabotla 2 1 Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, AP,-522510, India. 2 Department of Chemistry, Sir CR Reddy College, Eluru, AP-534007, India. 3 Department of Chemistry, Vignan Degree College, Guntur, AP-522009, India. Authors’ contributions This work was carried out in collaboration among all authors. Author SMT designed the study, performed the method development and validation, wrote the protocol, and wrote the first draft of the manuscript. Authors AV and SNMB helped the analyses of the study. Author KSPA managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i31B31707 Editor(s): (1) Dr. Prem K. Ramasamy, Brandeis University, USA. Reviewers: (1) Jean De Dieu Nzabonakuze, Lanzhou University, China. (2) Charles Nyandwi, Lanzhou University, China. (3) Atared Saad Jebur AL-Mashhadi, Al-Mustaqbal University College, Iraq. Complete Peer review History: http://www.sdiarticle4.com/review-history/68948 Received 01 April 2021 Accepted 09 June 2021 Published 15 June 2021 ABSTRACT Aims: The present application is a Newly Validated Reverse Phase-High Performance Liquid Chromatography Method for the Assay of Dexmethylphenidate and Serdexmethylphenidate with PDA. Study design: Mentioned study is a quick, rapid, economical, precise, and accurate reverse phase- high performance liquid chromatographic method for estimating Dexmethylphenidate and Serdexmethylphenidate. Place and duration of study: The present assay was carried out at the Shree icon Pharma Original Research Article