International Journal of Ayurvedic Medicine, Vol 13 (4), 2022; 939-943 Published online in http://ijam.co.in ISSN No: 0976-5921 Research Article Key Words: Venpucani, Aphrodisiac, Jaundice, Leucorrhoea, Pandanus odoratissimus. Introduction Venpucani ilakam is a Siddha polyherbal formulation consisting of 18 ingredients. It is prescribed for Manjal noy (Jaundice), Utal (edema), Enpu Vettai (Venereal diseases with bone manifestation), Piramiyam (Gonorrhea), Vellai (leucorrhoea), Nirc curukku (dysuria), Veppu noy (all types of fever) This drug is anabolic and aphrodisiac in nature. The therapeutic dosage is 5 to 10 g. Ethno pharmacological use of its ingredient Benincasa hispida Cogn is in leucorrhoea, fever, disease related to liver & edema (1), Pandanus odoratissimus Roxb in jaundice (2) edema (3) & dysuria (4) Piper nigrum in jaundice (5), edema (6), gonorrhea, leucorrhoea (7) & fever (8), Citrus aurantifolia in fever & jaundice (9), Glycyrriza glabra Linn. in edema (10), Quercus infectoria Oliv. in edema (11); anabolic nature of Myristica fragrans Houtt. (12) and aphrodisiac property of P. nigrum (7) & P. odoratissimus (4) validates the use of this drug in traditional medicine. The pharmacopoeial standards for this drug are not available for assessing the quality. As the demand for herbal medicine is tremendously increasing, the necessity for arriving pharmacopoeial parameter is greatly sensed by the world community. Hence, the physicochemical parameters for Ilagam, thin layer chromatographic photo documentations under ultra violet light at short and long wave lengths, white light after derivatization with 5 % sulphuric acid and high performance thin layer chromatographic finger print profiles were carried out for defatted chloroform extract and successive ethanol extract. Materials and Methods Procurement of Raw Drugs The ingredients of Venpucani ilakam their common name, botanical name, the anatomical part used and quantity required are listed in Table 1. All the raw drugs from Sl.No.6 to 16 were procured from raw drug market in Chennai and other ingredients were collected from Chengalpat. All the items were authenticated by the Pharmacognosist of this Institute. Preparation of VPI Juices of items Sl.No.1 to 4 were taken. Powdered the ingredients 7 to 16 and sieved through Pharmacopoeial Standards for Venpucani Ilakam - A classical Siddha medicine Achintya Kumar Mandal 1 , Shakila Ramachandran 2* 1. Assistant Research Officer (Chemistry), 2. Research Officer (Chemistry), Department of Chemistry, Siddha Central Research Institute (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai. Tamilnadu. India. Abstract Belief on the therapeutic values of herbal medicine is in increasing trend and the consumption of traditional medicine is also in upward trend. The quality control and assurance of these traditional medicines is a prime criterion for any herbal drug manufacturer. Venpucani ilakam (VPI) is a classical poly herbal Siddha medicine under the category Ilakam (Legiyam in Ayurveda). This poly herbal medicine is a combination of 18 ingredients of plants and animal origins. An attempt was made to prepare the drug with authenticated ingredients and subjected to physicochemical analysis, thin layer chromatographic photo documentation and high performance thin layer chromatographic finger printing. The TLC photo documentation of defatted chloroform extract showed nine spots under UV 254, ten spots under UV 366 nm and eleven spots after derivatization with vanillin sulphuric acid; defatted success ethanol extract showed five spots under short wavelength, six spots under long wavelength and seven spots after derivatization. HPTLC finger printing recorded eleven peaks under 254 nm, seventeen under 366 nm and fifteen peaks after derivatization in defatted chloroform extract; nine peaks under 254 nm, eleven peaks under 366 nm and eight peaks after derivatization in defatted ethanol extract. About twenty three percent loss in weight was observed on heating at 105 o C and drug was acidic nature. Not less than fourty percentage of the drug was soluble in alcohol and also in water. This drug has wide therapeutic use and the derived results may serve as reference standards. 939 * Corresponding Author: Shakila Ramachandran Research Officer (Chemistry), Department of Chemistry, Siddha Central Research Institute (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai. Tamilnadu. India. Email Id: rshakila@gov.in