ISPUB.COM The Internet Journal of Gynecology and Obstetrics Volume 23 Number 1 DOI: 10.5580/IJGO.53956 1 of 10 Correlation Among Follicle-stimulating Hormone, Insulin- like Growth Factor-I and Aromatase Expression with Oocyte Maturation in an Assisted Reproductive Program W Permadi, T Djuwantono, A Guyansyah, A Biben, J C Mose, K Mantilidewi, B S Hernowo, L Septiani, A Faried Citation W Permadi, T Djuwantono, A Guyansyah, A Biben, J C Mose, K Mantilidewi, B S Hernowo, L Septiani, A Faried. Correlation Among Follicle-stimulating Hormone, Insulin-like Growth Factor-I and Aromatase Expression with Oocyte Maturation in an Assisted Reproductive Program. The Internet Journal of Gynecology and Obstetrics. 2019 Volume 23 Number 1. DOI: 10.5580/IJGO.53956 Abstract Background: Follicular fluid plays important roles in oocyte quality, which can increase the success rate of assisted reproductive technology (ART) program. Oocyte maturity is influenced by the follicular fluid’s chemical component, including insulin-like growth factor (IGF)-I, which increases follicle-stimulating hormone (FSH) receptor; thereby increasing levels of intracellular cyclic adenosine monophosphate (cAMP) as primary mediator of FSH stimulation in aromatase expression. Materials and Methods: This was analytical correlational study with a cross-sectional approach in ART patients program at Dr. Hasan Sadikin Hospital Bandung. The p <0.05 considered as significance. Results: Mature and immature oocytes showed significant differences in FSH expression (p=0.03). The relationship of IGF-I and oocyte maturity showed no statistical significance (p=0.192). The median ratio of mature oocytes to total oocytes was 0.71; those with a median prevalence ratio > 0.71 showed strong FSH expression, which 1.71-fold higher than those with a lower proportion of mature oocytes. The aromatase expression in the follicular fluid of those patients with a median prevalence ratio of mature oocytes > 0.71 was 1.55-fold higher than in those with lower proportion of mature oocytes, who exhibited aromatase- negative-expression in the follicular fluid. Conclusion: Higher aromatase expression results in higher IGF-I and FSH expression in follicular fluid. INTRODUCTION Infertility rates continue to increase worldwide; based on demographic and health survey by the World Health Organization in 2012, the burden of infertility is still high in women. In the period from 1990-2010, the number of couples with infertility increased from 42 million to 48.5 million [1]. In a demographic and health survey from the period 1994-2000, the percentage of Indonesian women (aged 15-49 years) with infertility was approximately 10.2% [2]. The success of assisted reproductive technology (ART) as reflected by the rate of implantation, pregnancy and childbirth is still approximately 30% [3,4]. Follicle growth monitoring and reproductive hormonal level evaluation are integral parts of the ART program. The number and quality of oocytes reflects the success of the program. Therefore, the assessment of ovarian function to predict the number and quality of oocytes is very important [5]. Chemical elements of follicular fluid have been grouped into several categories, including a) hormones (follicle- stimulating hormone or FSH, leutinizing hormone or LH, human chorionic gonadotropin or hCG), b) growth factors of the transforming growth factor-beta (TGF-beta) superfamily, c) other growth factors (insulin-like growth hormone), and d) reactive oxygen species (ROS) [6]. Several animal studies have studied the expression of aromatase and chemical elements in follicular fluid as markers of oocyte quality, including FSH and insulin-like growth factor (IGF)-I [6,7], but until now, research on human follicular fluid has not