Natural Science: Journal of Science and Technology ISSN-p: 2338-0950 Vol 8 (1) : 20 – 27 (April 2019) ISSN-e: 2541-1969 Corresponding author: riskasari060@gmail.com 20 Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Penambahan Ekstrak Tomat Dan Air Kelapa Callus Induction of Potato (Solanum tuberosum L.) of c.v. Dombu In Vitro On Ms Medium With Additional Of Tomato Extract And Coconut Water Riska Sari *) , Asri P. Paserang, Ramadanil Pitopang, I Nengah Suwastika Jurusan Biologi, Fakultas MIPA, Universitas Tadulako, Palu ABSTRACT The research of In vitro callus induction in Dombu potato (Solanum tuberosum L) using the concentrations of tomato extracts and coconut water was carried out from May to September 2018 in the Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Sciences, Tadulako University. The research was aimed to know the influences of the concentrations of tomato extracts and coconut water against on to the formation of the callus in on Dombu potato (S. tuberosum L) by In vitro. This research was conducted with based on Completely Randomized Design (CRD) methods Rancangan Acak Lengkap (RAL) with seven treatments by which each treatment had four repetitions replication.and each culture bottle used and two explants on each botle. The treatment were: T1 (MS 0 + 10 % tomato extracts),T2 (MS 0 + 15 % tomato extracts), T3 (MS 0 +20 % tomato extracts), T4 (MS 0 + 20 % coconut water), T5 (MS 0 + 25 % coconut water), T6 (MS 0 +30 % coconut water), T7 (control/MS0). The results showed that the fastest callus formed in was on the treatment T6, was the fastest of allwith in average of 1.5 HST days after inoculation . The best media used for the growth of the callus was found on T7 (control). Keywords: Potato, Callus, Tomato Extracts, Coconut Water ABSTRAK Penelitian Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) secara In vitro dengan penambahan Konsentrasi Ekstrak Tomat dan Air Kelapa, telah diilaksanakan pada bulan Mei sampai September 2018 di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tadulako. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ekstrak tomat dan air kelapa terhadap pembentukan kalus tanaman kentang (S. tuberosum L.) secara in vitro. Penelitian ini dilakukan berdasarkan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan diulang sebanyak 4 kali. dan Dalam setiap satu botol kultur menggunakan ditanam 2 eksplan. Perlakuan yang dicobakan terdiri dari: T1 (MS 0 + ekstrak tomat 10 %), T2 (MS 0 + ekstrak tomat 15 %), T3 (MS 0 + ekstrak tomat 20 %), T4 (MS 0 + air kelapa 20 %), T5 (MS 0 + air kelapa 25 %), T6 (MS 0 + air kelapa30 %), T7 (Kontrol/MS0). Hasil penelitian menunjukkan bahwa Hari pembentukan kalus tercepat, yang terbentuk lebih cepat pada perlakuan T6 dengan rata-rata 1,5 HST. Media yang paling baik digunakan pada pertumbuhan kalus terdapat adalah pada T7 (Kontrol). Kata kunci: Kentang, Kalus, Ekstrak Tomat, Air Kelapa