© 2016. Fitsum Tessema, Fufa Abunna, Reta Duguma, Takele Beyene, Asmamaw Bihonegn, Teklemariam Worku, Biyansa Adugna & Mulualem Almazu. This is a research/review paper, distributed under the terms of the Creative Commons Attribution- Noncommercial 3.0 Unported License http://creativecommons.org/licenses/by-nc/3.0/), permitting all non commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Global Journal of Science Frontier Research: D Agriculture and Veterinary Volume 16 Issue 2 Version 1.0 Year 2016 Type : Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4626 & Print ISSN: 0975-5896 Drug Resistance Pattern of Staphylococcus in Poultry in Central and Southern Ethiopia By Fitsum Tessema, Fufa Abunna, Reta Duguma, Takele Beyene, Asmamaw Bihonegn, Teklemariam Worku, Biyansa Adugna & Mulualem Almazu Addis Ababa University, Ethiopia Abstract- This study was conducted from November 2014 - May 2015 to determine the antimicrobial resistance pattern of Staphylococcus species in poultry in Central and Southern Ethiopia. 205 staphylococcal species isolated from poultry were evaluated using disk diffusion method for their antimicrobial susceptibility to 10 different antimicrobial drugs. Staphylococcus were found to be highly susceptible to Ciprofloxacin (85.4%) followed by Sulfamethoxazole- Trimethoprim (68.8%). However these isolates were highly resistant to Penicillin G (94.1%) and Tetracycline (79%) followed by Amoxicillin (60.5%). From all Staphylococci isolates tested for drug susceptibility pattern, only 1 isolate (S. aureus) was susceptible to all tested drugs and 99.51% of isolates were resistant to at least one of the antibiotics tested. Coagulase negative Staphylococci were highly resistant to all tested drugs except Ciprofloxacin (0%) and S. aureus were highly resistant to Penicillin G (92.2%) and Tetracycline (74.5%). Staphylococcus species isolated in poultry in Central and Southern Ethiopia were all multidrug resistant. Keywords: drug resistance, ethiopia, poultry, staphylo-coccus. GJSFR-D Classification : FOR Code: 830309 DrugResistancePatternofStaphylococcusinPoultryinCentralandSouthernEthiopia Strictly as per the compliance and regulations of :