Citation: Masood, S.; Alkubaisi, N.A.; Aslam, M.; Salman, M.; Baraka, M.A.; Mustafa, Z.U.; Mallhi, T.H.; Khan, Y.H.; Meyer, J.C.; Godman, B. Knowledge of Human Monkeypox Infection among Final Year Medical, Pharmacy, and Nursing Students: A Multicenter, Cross-Sectional Analysis from Pakistan. Healthcare 2023, 11, 2777. https://doi.org/10.3390/ healthcare11202777 Academic Editor: Jose Granero-Molina Received: 4 September 2023 Revised: 22 September 2023 Accepted: 26 September 2023 Published: 20 October 2023 Copyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). healthcare Article Knowledge of Human Monkeypox Infection among Final Year Medical, Pharmacy, and Nursing Students: A Multicenter, Cross-Sectional Analysis from Pakistan Sahabia Masood 1 , Noorah A. Alkubaisi 2 , Maryam Aslam 3 , Muhammad Salman 4 , Mohamed A. Baraka 5,6, * , Zia Ul Mustafa 7,8, *, Tauqeer Hussain Mallhi 9 , Yusra Habib Khan 9 , Johanna C. Meyer 10 and Brian Godman 10,11,12 1 Department of Medicine, University Medical and Dental College Faisalabad, Faisalabad 38000, Pakistan; sahabiamasood15@gmail.com 2 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; nalkubaisi@ksu.edu.sa 3 Basic Health Unit (BHU), 554GB, Tehsil Tandlianwala, Faisalabad 38000, Pakistan; maryamaslam00999@gmail.com 4 Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore 54000, Pakistan; msk5012@gmail.com 5 Department of Pharmacy, Fatima College of Health Sciences, Abu Dhabi P.O. Box 3798, United Arab Emirates 6 Clinical Pharmacy Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11651, Egypt 7 Discipline of Clinical Pharmacy, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Gelugor 11800, Penang, Malaysia 8 Department of Pharmacy Services, District Headquarter (DHQ) Hospital, Pakpattan 57400, Pakistan 9 Department of Clinical Pharmacy, College of Pharmacy, Jouf University, Sakaka 72388, Saudi Arabia; thhussain@ju.edu.sa (T.H.M.); yhkhan@ju.edu.sa (Y.H.K.) 10 Department of Public Health Pharmacy and Management, School of Pharmacy, Sefako Makgatho Health Sciences University, Pretoria 0208, South Africa; hannelie.meyer@smu.ac.za (J.C.M.); brian.godman@strath.ac.uk (B.G.) 11 Strathclyde Institute of Pharmacy and Biomedical Science (SIPBS), University of Strathclyde, Glasgow G4 0RE, UK 12 Centre of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman P.O. Box 346, United Arab Emirates * Correspondence: mohamed.baraka2020@gmail.com (M.A.B.); ziaulmustafa@student.usm.my (Z.U.M.) Abstract: The World Health Organization (WHO) declared the monkeypox outbreak a public health emergency in June 2022. In Pakistan, positive cases of monkeypox were reported in April 2023. Healthcare workers (HCWs) are considered as a front-line force to combat such outbreaks. A questionnaire-based cross-sectional study was conducted among 11 public sector educational insti- tutions in Punjab, Pakistan, during May and June 2023 among final year medical, pharmacy, and nursing students concerning their knowledge of monkeypox. This included the signs/symptoms of monkeypox. Healthcare students were chosen as they are the HCWs of tomorrow. A total of 389 healthcare students participated in the study, with a mean age of 23.17 ± 1.72 years, and the majority were female. The mean knowledge score was 17.69 ± 4.55 (95% CI 17.24–18.14) out of a maximum total knowledge score of 26 (each correct answer was given a score of 1). The proportion of students with good, moderate, and poor knowledge was 21.6%, 43.2%, and 35.2%, respectively. Age (p = 0.017), gender (p < 0.001), and education (p < 0.001) had a significant impact on the knowledge score. In the multivariate linear regression model, education was the only significant factor linked to knowledge scores. Overall, the majority of future HCWs had moderate knowledge of monkeypox. Consequently, educational activities are needed to improve monkeypox-related knowledge among future HCWs. Furthermore, emerging infectious diseases should be routinely incorporated into HCW curricula. Keywords: outbreak; monkeypox; medical; knowledge; healthcare students; Pakistan Healthcare 2023, 11, 2777. https://doi.org/10.3390/healthcare11202777 https://www.mdpi.com/journal/healthcare