ORIGINAL PAPER Genomics-based high-resolution mapping of the BaMMV/BaYMV resistance gene rym11 in barley (Hordeum vulgare L.) Thomas Lu¨pken • Nils Stein • Dragan Perovic • Antje Habekuß • Ilona Kra¨mer • Urs Ha¨hnel • Burkhard Steuernagel • Uwe Scholz • Rounan Zhou • Ruvini Ariyadasa • Stefan Taudien • Matthias Platzer • Mihaela Martis • Klaus Mayer • Wolfgang Friedt • Frank Ordon Received: 5 September 2012 / Accepted: 13 January 2013 / Published online: 2 March 2013 Ó Springer-Verlag Berlin Heidelberg 2013 Abstract Soil-borne barley yellow mosaic virus disease, caused by different strains of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), is one of the most important diseases of winter barley (Hordeum vulgare L.) in Europe and East Asia. The recessive resis- tance gene rym11 located in the centromeric region of chromosome 4HL is effective against all so far known strains of BaMMV and BaYMV in Germany. In order to isolate this gene, a high-resolution mapping population (10,204 meiotic events) has been constructed. F 2 plants were screened with co-dominant flanking markers and segmental recombinant inbred lines (RILs) were tested for resistance to BaMMV under growth chamber and field conditions. Tightly linked markers were developed by exploiting (1) publicly available barley EST sequences, (2) employing barley synteny to rice, Brachypodium distach- yon and sorghum and (3) using next-generation sequencing data of barley. Using this approach, the genetic interval was efficiently narrowed down from the initial 10.72 % recombination to 0.074 % recombination. A marker co- segregating with rym11 was developed providing the basis for gene isolation and efficient marker-assisted selection. Introduction Barley yellow mosaic virus disease caused by different strains of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) (Huth 1991; Hariri et al. 2000, 2003; Kanyuka et al. 2004; Habekuss et al. 2008; Ku¨hne 2009) has become one of the most important dis- eases of winter barley in East Asia and in Europe (Ordon et al. 2004; Kanyuka et al. 2003). The area infested is continuously growing and high yield losses of susceptible winter barley crops are frequently observed in Europe. Due to transmission of the viruses via the soil-borne plasmo- diophorid Polymyxa graminis, breeding for resistant culti- vars is the only way to ensure winter barley cultivation in the growing area of infested fields. Up to now, resistance in Communicated by P. Hayes. Electronic supplementary material The online version of this article (doi:10.1007/s00122-013-2047-3) contains supplementary material, which is available to authorized users. T. Lu¨pken Á D. Perovic Á A. Habekuß Á I. Kra¨mer Á F. Ordon (&) Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany e-mail: frank.ordon@jki.bund.de N. Stein Á U. Ha¨hnel Á B. Steuernagel Á U. Scholz Á R. Zhou Á R. Ariyadasa Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany S. Taudien Á M. Platzer Leibniz Institute for Age Research, Fritz-Lipmann-Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany M. Martis Á K. Mayer Munich Information Center for Protein Sequences/Institute of Bioinformatics and Systems Biology (MIPS/IBIS), Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany W. Friedt Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany 123 Theor Appl Genet (2013) 126:1201–1212 DOI 10.1007/s00122-013-2047-3