Texila International Journal of Public Health ISSN: 2520-3134 DOI: 10.21522/TIJPH.2013.13.01.Art018 Received: 13.12.2024 Accepted: 17.01.2025 Published on: 28.03.2025 *Corresponding Author: drgkanbazhagan@gmail.com Anti-biofilm Effects of Resin-Modified Glass-Ionomers Incorporated with Silver Nanoparticles and Sodium Fluoride Kavya Gopalakrishnan 1 , Ajay Ramesh 2 , Abirami Devendran 3 , Sankarganesh Palaniyandi 4 , Lokesh Elumalai 5 , Krishnasamy Loganathan 6 , Pugazhenthi Elumalai 1 , Ganesh Kumar Anbazhagan 7 * 1 Department of Microbiology, Hindustan College of Arts & Science, Padur, OMR Chennai – 603 103, Tamil Nadu, India. 2 Department of General Surgery, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai – 602105. 3 Department of Microbiology, Vivekanandha College of Arts and Sciences for Women, Ellayampalayam, Tiruchengode 637 205, Tamil Nadu, India. 4 Department of Food Technology, Hindustan Institute of Technology and Science, Padur, Chennai – 603103, Tamil Nadu, India. 5 Department of Microbiology, Periyar University, Salem – 636 011, Tamil Nadu, India. 6 Department of Biotechnology, Hindustan College of Arts & Science, Padur, OMR Chennai – 603 103, Tamil Nadu, India. 7 Department of Microbiology, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai – 602 105, Tamil Nadu, India. Abstract The glycocalyx or biofilm is essential for microbial survival. But dental biofilms cause cavities in teeth. The current study planned to incorporate silver nanoparticles and sodium fluoride in resin- modified glass-ionomer cement to test anti-biofilm efficacy. Silver nanoparticles (NAg) were chemically reduced by sodium borohydride. At 0.15% mass fraction, NAg was added in commercial resin-modified glass-ionomer cement (GC) and in this blend, 4 mass fractions (5%, 10%, 20%, and 30%) of NaF were mixed. The metabolic activity of biofilm, which shows the metabolic activity was decreased by NaF (P > .05). The biofilm CFU count shows the CFU counts of GC+NAg+20%NaF were decreased to 10 6 CFU/disk (P<.05). The cell viabilities of GC+NAg+20%NaF were79.7%,75.6%,73% for days 1, 4, and 7 respectively, which were lower than GC control (P < .05) but higher than GC+NAg (P < .05). The use of NAg reduced cell viability at all three-time points (day 1, day 4, and day 7), which was partially reversed by the addition of NaF. Keywords: Biofilm, Glass-Ionomer, Orthodontic Cement, Silver Nanoparticles, Sodium Fluoride. Introduction The advancement from tying down orthodontic connections to teeth using circumferential groups to theimmediate holding of individual orthodontic sections has happened during the beyond 35 years. Dental caries is the most prevalent and multifactorial oral disease and is still considered a serious worldwide oral health problem. Antibiotics, metal ions, and fluorides in various vehicles have been used as alternatives to the traditional filling approach for preventing and arresting caries. Topical agents, such as silver diamine fluoride (SDF), have been concluded as effective, efficient, equitable, and safe caries-preventive agents. SDF therapy is simple and non-invasive [1]. Clinical trials and lab studies have supported the success of 38% SDF in arresting dental