28 days as compared to controls. Simultaneous exposure to FSS and LCT for 28 days in rats resulted to marked increase plasma corticosterone levels, blood brain barrier permeability and decreases motor activity, DA-D2 re- ceptors, expression of tyrosine hydroxylase and alteration in levels of DA, DOPAC and HVA in corpus striatum as compared to rats in either FSS, LCT or control groups. It was interesting to note that simultaneous exposure to FSS and LCT treatment also caused a marked decrease in mitochondrial dysfunctions associated with decreased the expression of anti-apoptotic while pro- apoptotic and stress marker proteins was enhanced in corpus striatum of these rats as compared to either FSS, LCT or in control group. The results exhibit that physical stresses contribute in the LCT induced dopaminergic alterations associated with mitochondrial dysfunctions. P-024 DEVELOPMENT OF NANO-FORMULATION CONTAINING RUTIN FOR THE PROTECTIVE AND BENEFICIAL EFFECT AGAINST 6-HYDROXYDOPAMINE INDUCED PARKINSON’S DISEASE MODEL VIA ALTERED THE GENETIC BACKGROUNDS V. Sahai, V. Kumar. Pharmacy, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, India Objective: Parkinson’s disease (PD) characterized by the interaction be- tween the number of factors viz., mitochondrial deformity, genetics toxins, aging, and oxidative stress. Various evidences suggest that regu- lar con- sumption of an antioxidant rich food may alter the incidence of neuro- degenerative diseases. The aim of the current study was to prepare and scrutinize the nanoherbaceutical formulation containing rutin against 6- hidroxidopamine (6-OHDA) induced PD animal model. Material & methods: Top-down technique was used for the preparation of nanoherbaceutical suspen- sion. Intracerebroventricular injection of 6- OHDA was used for induction the PD and mice were treated with the nano- formulation of rutin for 56 days. The enzymatic activities viz., Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH), glutathione S-transferase (GST) and level of reactive oxygen species (ROS), total reactive antioxidant dopamine and its metabolites homo- vanillic and 3,4-dihydroxyphenylacetic acid, were scrutinized in the striatum. Behavioural parameters including locomotor, memory and depressive were scrutinized, respectively. Result & discussion: Spectroscopy approaches clearly showed that development of rutin loaded nano- formulation. The RT-PLGA-NP treat- ment was proficient in averting the memory destruction in Morris water maze test as well as depressive like behaviour in tail suspension test. Nano-formulation rutin attenuated 6-OHDA induced down-regulation in total reactive antioxidant, CAT and GPx, dopamine and its metabo- lite level in striatum of mice. We also observed the up-regulation levels of GR and reactive oxygen species in the striatum and these modulations were diminished by nano-formulation of rutin. Conclusion: Collectively, we can conclude that nanoformulation of rutin showed a beneficial effect on 6-OHDA induced neurotoxicity in mice, signifying that it could be alternative therapy for the treatment of PD. P-025 EFFECT OF ROPINIROLE ALGINATE NANOCOMPOSITE (RANC) ON THE TRANSGENIC DROSOPHILA MELANOGASTER MODEL OF PARKINSON’S DISEASE F. Naz, A. Fatima, R. Rahul, S. Jyoti, Y. Hasan Siddique. Zoology, Aligarh Muslim University, Aligarh, India Objective: In the present study the effect of ropinirole alginate nano- composite (RANC) was studied on the transgenic Drosophila expressing human alpha synuclein. Methods: RANC was synthesized having 1, 2, and 3mM of ropinirole and characterized by TEM, SEM and FTIR. The RANC was mixed in diet of Drosophila to establish the final concentrations of 1, 2 and 3mM of ropi- nirole. The PD flies were allowed to feed on it for 30 days. After 30 days of exposure the flies were as- sayed for climbing assay, activity pattern, oxidative stress markers, caspase-3 & 9 activity and dopamine content. Results: A dose dependent significant delay in the loss of climbing ability and improvement in the activity pattern was observed in PD flies. A dose dependent decrease in the oxidative stress markers and increase in dopamine content was observed in the PD flies exposed to various doses of RANC. The PD flies exposed to RANC showed more improvement in comparison to the PD flies exposed to only ropinirole. Conclusions: It is concluded that the drugs in the form of nanocomposite could play a pivotal role as they will reduce the toxicity/side effects associated with the excessive use or high dose of the drug to treat vari- ous central nervous system disorders. P-026 EVALUATION OF THE THERAPEUTIC POTENTIAL OF TANGERITIN AGAINST THE SYMPTOMS OF PARKINSON’S DISEASE A. Fatima, F. Naz, R. Rahul, Y.H. Siddique. Zoology, Aligarh Muslim University, Aligarh, India Parkinson’s disease (PD) is a progressive neurodegenerative disorder. The disease is characterised by the loss of dopaminergic neurons leading to the major pathological hallmarks. Objective: The study aims to evaluate the effect of the dietary supple- mentation of tangeritin on the Drosophila model of PD and also to study the antioxidant potential of tangeritin. Methods: Tangeritin at a final concentration of 5, 10 and 20mM was added in the diet and the flies were allowed to feed on it for 24 days. At the same time other set of PD flies were allowed to feed on a diet hav- ing 10 -3 M of L-Dopa. The effect of tangeritin was studied on life span, activity pattern, olfactory deficiency, fecundity assay, retinal degeneration and oxidative stress markers. The antioxidant potential of the flavo- noid was evaluated using the 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scav- enging activity, superoxide anion scavenging activity and nitrite scav- enging activity. Results: The exposure of PD flies to tangeritin showed significant increase in the life span, improvement in activity and prevents retinal degeneration. Tangeritin also showed free radical as well as superoxide and nitrite scavenging activity. Conclusions: The results of the present study conclude that tangeritin, due to its antioxidant property, could play an important role in the treatment of Parkinson’s disease. P-027 MOLECULAR MECHANISMS OF NEURODEGENERATION AND NEUROPLASTICITY IN THE NIGROSTRIATAL SYSTEM AT MODELING PARKINSON’S DISEASE AS AN INSTRUMENT FOR TRANSLATIONAL MEDICINE E. Mingazov 1 , G. Khakimova 1 , A. Kolacheva 1 , E. Kozina 1 , A. Medvedev 2 , A. Bazyan 3 , M. Ugrumov 1 . 1 Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology RAS, Moscow, Russian Federation; 2 Laboratory of Pharmacoproteomics, Institute of Biomedical Chemistry RAS, Moscow, Russian Federation; 3 Laboratory of Neurochemical Mechanisms of Learning and Memory, Institute of Higher Nervous Activity and Neurophysiology RAS, Moscow, Russian Federation Objective: Parkinson’s disease (PD) is characterized by the appearance of motor symptoms many years after the onset of neurodegeneration, which explains low efficiency of therapy. The objective of our work was to study molecular mechanisms of neurodegeneration and neuroplasticity in the nigrostriatal system at modeling preclinical and clinical stages of PD that would serve to develop preclinical diagnosis and preventive therapy. Methods:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice at presymptomatic and symptomatic stages of parkinsonism were used to evaluate (i) the number of dopaminergic neurons with immunohistochemistry; (ii) catecholamine content with HPLC; (iii) content of specific proteins and mRNAs by Western-blot and real-time PCR; Abstracts / Parkinsonism and Related Disorders 46 (2018) e38ee46 e43