Andrologia. 2020;00:e13555. wileyonlinelibrary.com/journal/and | 1 of 5 https://doi.org/10.1111/and.13555 © 2020 Blackwell Verlag GmbH 1 | INTRODUCTION Cryopreservation is a reversible process that brings sperm metabolism to a standstill (Holt, Penfold, Chenoweth, & Lorton, 2014). Therefore, it is possible to store genetic materials. However, cryopreservation can induce detrimental effects (ice crystallisation, cold shock, lipid perox- idation, etc; Nur, Zik, Ustuner, Sagirkaya, & Ozguden, 2010). These undesirable effects may cause irreversible decrease on sperm func- tion parameters (Nur et al., 2010; Ustuner et al., 2016). The success of sperm cryopreservation depends on the composition of the extenders which is a crucial factor to protect spermatozoon (Alcay, Gokce, et al., 2016; Toker, Alcay, Gokce, & Ustuner, 2016). Lecithin has an important effect on the regulation of animal cells’ bio-membrane (Gokce, Alcay, & Gul, 2017; Ustuner, Alcay, Nur, Sagirkaya, & Soylu, 2014). Therefore, it is used for sperm cryopres- ervation either as an extracted substance or as an ingredient of egg yolk (Toker et al., 2016; Ustuner et al., 2014). Lecithin protects the sperm plasma membrane by restoring the phospholipids lost during the heat shock (Toker et al., 2016). Many researchers have obtained acceptable semen parameters with using lecithin-based extenders at post-thaw and after incubation (Forouzanfar et al., 2010; Najafi, Daghigh-Kia, Dodaran, Mehdipour, & Alvarez-Rodriguez, 2017; Toker et al., 2016; Ustuner et al., 2014). Seminal plasma contains biochemical components that regulate sperm function (Ciereszko, Glogowski, & Dabrowski, 2000). The use of seminal plasma of different species in the semen extenders has been tested to minimise the detrimental effects of cryopreser- vation (Baran, Ak, Ileri, & Soylu, 2004; Gunay et al., 2006; Ustuner et al., 2016). Rainbow trout seminal plasma (RTS) contains proteins (2.1 ± 0.3 mg/ml), monosaccharides, amino acids, free fatty acids, phospholipids, vitamins and neutral lipids (Ciereszko et al., 2000; Shaliutina-Kolešová et al., 2016). In addition, RTS supplemented Received: 16 October 2019 | Revised: 20 December 2019 | Accepted: 4 February 2020 DOI: 10.1111/and.13555 ORIGINAL ARTICLE Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders Selim Alcay 1 | Burcu Ustuner 1 | Ahmet Aktar 1 | Emine Mulkpinar 1 | Muhammed Duman 2 | Mustafa Akkasoglu 1 | Mehmet Cetinkaya 1 1 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey 2 Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey Correspondence Selim Alcay, Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey. Email: salcay@uludag.edu.tr Abstract The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post- thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin- based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 10 6 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA-FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post-thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma mem- brane functional integrity and mitochondrial function better than the control group ( p < .05). The study demonstrated that RTS supplemented lecithin-based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender. KEYWORDS post-thaw incubation, saanen goat, seminal plasma, sperm freezing