Middle East Journal of Applied Science & Technology (MEJAST) Volume 7, Issue 4, Pages 103-105, October-December 2024 ISSN: 2582-0974 [103] Assessment of Co-relation between Sleeping Time and Blood grouping Muhammad Imran Qadir 1* & Mahnoor Ilyas 2 1,2 Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan. Corresponding Author (Muhammad Imran Qadir) Email: mrimranqadir@hotmail.com * DOI: https://doi.org/10.46431/MEJAST.2024.7409 Copyright © 2024 Muhammad Imran Qadir & Mahnoor Ilyas. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Article Received: 12 October 2024 Article Accepted: 14 December 2024 Article Published: 26 December 2024 ░ 1. Introduction ABO blood group is type of multiple alleles. ABO blood group system was firstly discovering by Karl Land Steiner. ABO blood group has four different phenotypes which distinct from each other due to presence of specific antigen on surface of RBCs. If a person having antigen A its blood group is A. While a person having antigen B then its blood group is B. If a person having both antigen A and B on RBCs then blood group will be AB. A person having no antigen on RBCs then its blood group is O. ABO blood group system is encoded by a single polymorphic gene I on chromosome 9 and A phenotype having contains antibodies B in serum and agglutinate any RBCs with antigen A, while B phenotype contains anti-A antibodies in serum and phenotype AB neither anti A nor anti B antibodies in serum. ABO blood group is universal recipient while phenotype O absorbed by blood stream of recipient [1]. Rh blood group is defined on basis of Rh factor. I t is special type of protein present on red blood cell. ABO blood group is differentiating into positive and negative sign. Rh blood group system encodes three types of genes C, D, E. If a person having Rh factor on red blood cells then its blood group is positive respectively A+, B+, AB+ and O+. While Rh factor is not present on red blood cells of person then its blood group is negative. Basically it has been seen in rhesus monkey [2]. Sleep is a naturally recurring state of body and mind, characterized by consciousness relatively inhibited by sensory activity. Sleep occurs in repeating periods in which the body alternates between two distinct modes REM and non REM. Although it helps in anabolic state, helps to restore immune nervous, muscular and skeleton system these are vital process that cognitive function plays large role in the function of internal endocrine and immune system. The internal circadian clock promotes daily sleep at night. Human suffers from various sleeping disorders. Many people have different sleeping time [3]-[5]. 1.1. Study Objectives Objective of present studies were: (1) To determine the ABO blood grouping; (2) To determine the Rh blood grouping; (3) To know the sleeping time; (4) To show influence of blood group on sleeping time; (5) To know the any difference of blood grouping affiliated sleeping time between male and female. ABSTRACT Objective of present questions related to sleeping time then we collect data accordance to their blood group. It was concluded from present studies that influence of blood group on sleeping time was maximum for A+ blood group and minimum for A- and AB- . Authors took sample of blood from person that posse’s different blood group and then finding out blood group through this previous technique. Total 177 subject s were participated in this studies all these were students of Bahauddin Zakariya University from 18 to 22 age group. A questionnaire was prepared about sleeping time. Keywords: Sleeping time; Phenotype; Rh factor; Red blood cells; Physical health; Mental health; Antigen A; Antigen B; Night; Fatigue.