SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1 in pulmonary epithelial and endothelial cells Magdalena Ambrożek-Latecka 1 , Piotr Kozlowski 2 , Grażyna Hoser 1 , Magdalena Bandyszewska 1 , Karolina Hanusek 3 , Dominika Nowis 4 , Jakub Gołąb 4 , Małgorzata Grzanka 3 , Agnieszka Piekiełko-Witkowska 3 , Luise Schulz 5 , Franziska Hornung 5 , Stefanie Deinhardt- Emmer 5 , Ewa Kozlowska 6 , Tomasz Skirecki 1* 1 Department of Translational Immunology and Experimental Intensive Care, Centre of Translational Research, Centre of Postgraduate Medical Education, Warsaw, Poland 2 Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland 3 Department of Biochemistry and Molecular Biology, Centre of Translational Research, Centre of Postgraduate Medical Education, Warsaw, Poland 4 Department of Immunology, Medical University of Warsaw, Warsaw, Poland 5 Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, Germany 6 Department of Immunology, Institute of Functional Biology and Ecology, Faculty of Biology, University of Warsaw, Warsaw, Poland Correspondence: Tomasz Skirecki Marymoncka 99/103 01-813 Warsaw Poland tskirecki@cmkp.edu.pl . CC-BY-ND 4.0 International license perpetuity. It is made available under a preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in The copyright holder for this this version posted November 14, 2023. ; https://doi.org/10.1101/2023.11.13.566917 doi: bioRxiv preprint