Adenovirus expressing CRTC2 or EGFP, and micro RNA (miRNA) knockdown approaches for CRTC2 were performed, then we used various assay. We have examined angiogenesis process by aorta ring assay in CRTC2 deficient mice. Results: BBMC plated on the Matrigel formed capillary like structures. Overexpressing CRTC2 had no effect on the pCREB protein level, CRTC2 enhanced tube formation and migration and CRTC2 knockdown using miRNA reduced angiogenesis. CRTC2 had no effect on proliferation, but overexpressing CRTC2 showed low paracellular permeability, that is, enhanced endothelial function. Conclusion: In brain microvascular endothelial cells, CRTC2 has essential role for angiogenesis. doi:10.1016/j.jns.2017.08.2431 2401 WCN17-1040 SHIFT 6 - STROKE Keeping head position flat reduces progressive motor paralysis of branch atheromatous disease (BAD) patients K. Kano, T. Saito, S. Takeguchi, K. Takahashi, S. Jun, T. Katayama, N. Hasebe. Asahikawa Medical University, Division of Neurology- Department of Internal Medicine, Asahikawa, Japan Background: Progressive motor paralysis appears frequently in patients with branch atheromatous disease (BAD). Keeping head position flat might reduce neurological deterioration through improvement of cerebral blood flow in early stage of BAD, however there are few detailed reports. Objective: To identify the effectiveness of keeping head position flat for BAD patients in the acute stage. Patients and Methods / Material and Methods: We retrospectively enrolled 87 consecutive patients diagnosed with BAD by stroke specialists of our hospital between 2010 and 2017. We define neurological deterioration as a decrease of Manual Muscle Test (MMT) score of extremities more than one point within 7 days of onset. Results: The patients were divided into 2 groups, 23 with keeping head position flat (HF group) and 64 with not keeping head position flat (non-HF group). The number of patients with progressive motor paralysis in HF group was 3 (13.0%) and significantly smaller than non-HF group (24, 37.5%; OR, 0.25; 95% CI, 0.07-0.88; P=0.036). All neurological deterioration appeared within 5 days of onset. The Kaplan-Meier curve showed that motor paralysis developed more progressively in non-HF group than HF group. Logistic regression analysis revealed that keeping head position flat solely was associated with a better neurological prognosis for BAD. Conclusion: This is the first report to clarify that keeping head position flat reduces neurological deterioration of BAD patients to 1/ 4. We recommend keeping BAD patients’ head position flat for at least 5 days from onset. doi:10.1016/j.jns.2017.08.2432 2402 WCN17-2381 SHIFT 6 - STROKE Acute ischemic stroke after recovery of cervical necrotizing lymphadenitis presumably caused by cat-scratch disease – A case report H.J. Lin. Chi-Mei Medical Center, Department of Neurology, Tainan, Taiwan, R, .O.C. Background: Cat-scratch disease is a generally benign infectious disease caused by Bartonella henselae, and commonly presents as regional lymphadenopathy. Objective: We reported an immunocompetent patient with unex- pected stroke complications. Patients and Methods / Material and Methods: A 35-year-old healthy woman experienced fever and progressive painful swelling over the right upper neck 5 days after a scratch on the upper back by her pet cat. She was hospitalized 2 days after the onset of the symptoms. Neck CT scans revealed enlarged lymph nodes and core needle biopsy showed necrotizing granulomatous inflammation. Under the diagnosis of cat- scratch disease, she was treated with doxycycline with good responses, and was discharged free of symptoms 11 days later. Two months after the discharge, she began to experience persistent mild headache with intermittent dizziness. In the following month, there were two episodes of severe dizziness with left hemiparesis, each lasting for 10 minutes and recovering spontaneously. One morning she woke up with more severe weakness in the left limbs and immediately presented to the emergency room, where left sensory-motor hemiparesis, left hemianopia, and left neglect syndrome were noted. Brain MRI and MRA demonstrated acute infarction in the right fronto-parietal areas and occlusion in the right extracranial internal carotid artery. Work-up for stroke etiologies including cardiology, biochemistry, hematology, and autoimmune diseases were unremarkable. She was prescribed aspirin for secondary prevention and the neurologic deficits improved gradually. Results Conclusion: Cat-scratch disease with severe cervical lymphadenitis may lead to an occlusive extracranial carotid arteritis with subse- quent cerebral thromboembolism. doi:10.1016/j.jns.2017.08.2433 2403 WCN17-2591 SHIFT 6 - STROKE 12/15 lipoxygenase inhibition suppresses neuroinflammation in a thrombotic permanent cerebral ischemia model C. Çakır-Aktaş a , M. Yemisci b , E. Bodur a , E. Eren-Kocak c , K. van Leyen d , T. Dalkara b , H. Karatas Kursun e . a Hacettepe University, Faculty of Medicine- Department of Biochemistry, Ankara, Turkey; b Hacettepe University, Institute of Neurological Sciences and Psychiatry- Faculty of Medicine- Department of Neurology, Ankara, Turkey; c Hacettepe University, Institute of Neurological Sciences and Psychiatry- Faculty of Medicine- Department of Psychiatry, Ankara, Turkey; d Massachusetts General Hospital, Dept. of Radiology- Neuroprotection Research Laboratories, Boston, USA; e Hacettepe University, Institute of Neurological Sciences and Psychiatry, Ankara, Turkey Background: Lipoxygenases (LOX) play important roles in stroke. 12/ 15-LOX inhibition reduces the infarct size and brain edema in acute phase of experimental ischemia; in the late phase it promotes axonal regeneration and revascularization; hence functional recovery. However, the effect of 12/15-LOX on ischemia-induced inflammation is not clarified. Objective: In this study, our aim was to investigate the effects of recently synthesized 12/15 LOX inhibitor (ML351) on neuroinflam- mation and blood brain barrier integrity in acute and subacute phase of experimental stroke. Patients and Methods / Material and Methods: To mimic human stroke, thrombotic MCA occlusion induced by FeCl 3 (permanent ischemia) was performed in mice. 12/15-LOX inhibitor (50mg/kg) or DMSO was injected intraperitoneally one-hour after ischemia. Mice were sacrificed 6, 24 and 72hrs after ischemia. Immunohistochemical Abstracts / Journal of the Neurological Sciences xxx (2017) 757–944 863