JURNAL ILMIAH PETERNAKAN TERPADU Vol. 8(1): 7 - 13, Maret 2020 Yulfia N. Selan et al. Terakreditasi Peringkat 3 Kemenristekdikti RI No. 21/E/KPT/2018 ISSN: 2614-0497 ©JIPT 8(1): 7 - 13, Maret 2020 7 HISTOMORFOLOGI DAN DISTRIBUSI KARBOHIDRAT NETRAL PADA ESOFAGUS DAN PROVENTRIKULUS AYAM HUTAN MERAH (Gallus gallus) ASAL PULAU TIMOR Histomorphology and Distribution of Neutral Carbohydrates in Esophagus and Proventriculus of Red Jungle Fowl (Gallus gallus) from Timor Island Yulfia N. Selan 1 *, Filphin A. Amalo 1 , Inggrid T. Maha 1 , Herlina U. Deta 2 , Aplonia B. Y. Teme 3 1 Laboratory of Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Nusa Cendana 2 Laboratory of Clinical, Reproductive, Pathology and Nutrition, Faculty of Veterinary Medicine, University of Nusa Cendana 3 Faculty of Veterinary Medicine, University of Nusa Cendana Jl. Adi Sucipto Penfui No.85001, Lasiana, Klp. Lima, Kupang, Nusa Tenggara Timur *E-mail: yulfia.nelly@gmail.com Submitted : October 30, 2019 Accepted : March 27, 2020 ABSTRAK Ayam hutan merah merupakan salah satu satwa endemik di Pulau Timor yang telah mengalami penurunan populasi. Penelitian ini bertujuan untuk mengetahui struktur histologi dan distribusi karbohidrat netral pada esofagus dan proventrikulus ayam hutan merah (Gallus gallus). Bahan utama penelitian ini berupa potongan tiga sampel jaringan esofagus dan proventrikulus. Ayam dimatikan dengan dislokasi servikal untuk mengambil organ esofagus dan proventrikulus yang difiksasi dalam formalin 10%. Struktur histomorfologi organ diidentifikasi dengan pewarnaan hematoksilin-eosin (HE) dan distribusi karbohidrat netral dengan pewarnaan Periodic Acid Schiff (PAS). Hasil penelitian menunjukkan struktur histomorfologi esofagus terdiri empat lapisan yaitu tunika mukosa, tunika submukosa, tunika muskularis, dan tunika adventisia. Struktur histomorfologi pada proventrikulus terdiri empat lapisan, yaitu tunika mukosa, tunika submukosa, tunika muskularis, dan tunika serosa. Distribusi karbohidrat netral pada kelenjar esofagus menunjukkan reaksi positif kuat sedangkan lamina epitel serta jaringan ikat longgar di lamina propria dengan reaksi positif lemah, dan pada proventrikulus terlihat reaksi positif kuat pada sel- sel yang melapisi epitel dan rongga kelenjar tubular sederhana. Kata kunci: Ayam Hutan Merah, Esofagus, Proventrikulus, Karbohidrat netral. ABSTRACT Red jungle fowl is one of the endemic animals in the island of Timor which has a decline in population. This study aimed to determine the histological structure and distribution of neutral carbohydrates in the esophagus and proventriculus of red jungle fowl (Gallus gallus). The main samples used in this study were three samples esophageal tissue and proventriculus. Chickens were killed by cervical dislocation to collection esophageal and proventricular organs those fixed in 10% formalin. The histolomorphology structures were identified by using hematoxylin-eosin (HE) staining and neutral carbohydrate distribution was identified by using Periodic Acid Schiff (PAS) staining. The results showed that esophageal histolomorphology structure consisted of four layers, namely tunica mucosal, tunica submucosal, tunica muscularis and tunica adventitia. Proventricular histolomorphology structure consisted of four layers, namely tunica mucosal, tunica submucosal, tunica muscularis and tunica serous. Neutral carbohydrate distribution were found in the esophageal glands showed strong positive reaction while epithelial lamina and loose connective tissue in the lamina propria showed weak positive reaction and proventriculus showed strong positive reactions in cells lining the epithelium and cavity of simple tubular glandular. Keywords: Red Jungle Fowl, Esophagus, Proventriculus, Neutral carbohydrate.s