ABSTRACTS Heart, Lung and Circulation Abstracts S239 2012;21:S143–S316 CSANZ 2012 Abstracts Results: At baseline IVUS-derived luminal CSA was (8.75 ± 0.26 mm 2 ) and MRI (8.71 ± 0.20 mm 2 ). Follow- ing GTN infusion IVUS-derived luminal CSA was (9.72 ± 0.18 mm 2 ) and MRI (9.90 ± 0.24 mm 2 ). The percent- age increase in luminal CSA was numerically, but not statistically significantly, higher with MRI (11.96%) than IVUS (9.94%). The correlation between IVUS and MRI at baseline and following GTN were excellent (intraclass cor- relation coefficient = 0.964 and 0.957). Conclusion: MRI is a feasible non-invasive modal- ity for the assessment of luminal CSA changes in very small calibre vessels. Accordingly, there is potential for MRI to provide endothelial function assessment in small central conduit vessels such as human coronary arteries. http://dx.doi.org/10.1016/j.hlc.2012.05.587 577 Recombinant Fibrillin-1 Fragment, PF8, a New Candidate to Biofunctionalise Endovascular Stents and Grafts H. Mollahajian 1,3,∗ , S. Wise 1 , P. Bannon 2,3 , M. Ng 1 1 The University of Sydney-The Heart Research Institute, Australia 2 The University of Sydney, Australia 3 The Baird Institute, Australia Background: Current vascular biomaterials exhibit poor biocompatibility characterised by excessive throm- bogenicity, failure to promote endothelialisation and predisposition to neoinitmal hyperplasia. Fibrillin-1, a major constituent of microfibrils is associated with elastin fibres in the arterial wall. Fibrillin-1 can attach to endothe- lial cells through a RGD cell adhesion motif, which is included in PF8, a recombinant fibrillin-1 fragment. We investigated the suitability of PF8 as a candidate protein to biofunctionalise vascular devices through facilitation of endothelialisation. Methods and results: PF8 was extracted from the transfected HEK-293 cell lines. Human Coronary Artery Endothelial Cells (HCAECs) and Human Umbilical Vein Endothelial Cells (HUVECs) were cultured on tissue cul- ture plastic coated with different proteins. PF8 showed remarkable adhesion to endothelial cells at low concentra- tion (77 ± 5.1%, 81 ± 1.8% and 87 ± 1.4% cell attachment at 0.75, 1.50 and 6.25 g/ml, respectively). In a comparative attachment study, PF8 showed superiority to fibronectin and tropoelastin, both well-known adhesive proteins for facilitating EC attachment by 33% (p < 0.01) and 63% (p < 0.001), respectively. PF8 also provided a better plat- form for proliferation of endothelial cells compared to fibronectin after three and five days (p < 0.01). When added to stainless steel (SS) and PTFE, PF8 showed protection against clot formation after exposure to whole blood for 30 min (Fig. 1). Conclusion: Recombinant PF8 enhances endotheliali- sation and confers thromboresistance in vitro. PF8 can be considered a suitable candidate for biofunctionalisation of endovascular devices. Figure 1. Effect of PF8 on thrombogenicity of SS and PTFE. http://dx.doi.org/10.1016/j.hlc.2012.05.588 578 Smart Phone and Tablet PC Use by the Medical Profession—A Vital Clinical Tool or a Dangerous Distrac- tion? “SMart Phone Utilisation for Rational Fact Finding” D. Platts 1,2,∗ , M. Brown 1,2 , G. Javorsky 1 , G. Scalia 1,2 , S. MacKenzie 1,2 1 The Prince Charles Hospital, Australia 2 School of Medicine, University of Queensland, Australia Background: There is a high uptake in the use of Smart phones (SMP) and tablet PCs (TPC) by the medical pro- fession. This study was performed to assess the usage, benefits and limitations of these devices as rated by car- diologists, cardiac surgeons, basic and advanced trainees and critical care staff, in the delivery of healthcare. The “SMURFF” Trial. Methods: Participants were invited via email to com- plete an anonymous, non-incentive, online survey (Survey Monkey TM ) with multichoice questions, covering a broad range of issues regarding this technology. Participants were from a large tertiary referral cardiac hospital and numerous private practices. This survey was approved by the hospital’s Human Research Ethics Committee. Results: Response rate 31% (44 staff:cardiology = 82%, critical care = 11%, cardiothoracic surgery = 7%). Eighty- four percent had either a SMP or TPC (Apple = 91%). Seventy-three percent had medical apps (46% had three or more installed). Forty-six percent use a medical app every day or every few days. Eighty-seven percent used a device to access medical information on the inter- net. Niinety-five percent use a device to communicate with colleagues on work related issues. Sixty-five per- cent found the photo/send function useful. Seventy-three percent found a device beneficial during clinical duties. The commonest reason for device interaction was access to internet for search engines (43%). Fifty-one percent reported stored patient data a privacy issue. Sixty-five per- cent did not think SMP/TPC cause distraction from clinical duties. The majority thought formal education should be considered for these devices. Conclusion: SMP and TPC are regularly used by the staff surveyed with the majority reporting positive benefits