Jagajothi A / Afr.J.Bio.Sc. 6(6) (2024) ISSN: 2663-2187 https://doi.org/ 10.33472/AFJBS.6.6.2024.1205-1212 Green synthesis and characterization of Terminalia chebula seeds mediated ZnO nanoparticles and their Larvicidal activity Jagajothi A. 1 , Srinivasan, K. 2 , Bhuvaneshwari, S. 3 , Rathika, R. 4* Suganthi, P. 5 1 Associate Professor, PG & Research Department of Zoology, Government Arts College (Autonomous), Salem – 636 007, Tamil Nadu, India 2 Guest Lecturer, PG & Research Department of Zoology, Government Arts College, Dharmapuri – 636705, Tamil Nadu, India 3 Assistant Professor, Department of Zoology, Bishop Heber College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, India 4 Associate Professor, PG & Research Department of Zoology, Government Arts College, Dharmapuri – 636705, Tamil Nadu, India 5 Senior Scientist, KIRND Institute of Research and Development Pvt. Ltd., Tiruchirappalli – 620020, Tamil Nadu, India Corresponding Author* Dr. R. Rathika Associate Professor, PG & Research Department of Zoology, Government Arts College, Dharmapuri – 636705, Tamil Nadu, India Article Info Volume 6, Issue 6, 2024 Received: 03 March 2024 Accepted: 30 April 2024 doi: 10.33472/AFJBS.6.6.2024.1205-1212 Abstract Green synthesis of nanoparticles can be an important alternative compared to conventional physiochemical synthesis. Terminalia chebula seed aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (ZnO NPs). Further, the synthesized ZnO NPs were subjected to various characterization techniques for their functional group and particle size as 37nm. Larvicidal activity of green synthesized ZnO NPs were tested against Anopheles third instar larvae showed significant LC50 value of 85ppm with regression coefficient R2 as 0.91. Our study concluded that T. chebula seed mediated ZnO NPs showed an effective larvicidal activity against malarial vector Anopheles third instar. Keywords:ZnO NPs, Terminalia chebula, FTIR, UV, XRD, Larvicidal activity