_____________________________________________________________________________________________________ *Corresponding author: Email: reshmimicro@srcw.ac.in; Cite as: Gopalakrishnan, R., & Selvi, B. T. (2024). Optimization Studies of Anticancer Enzyme -asparaginase from Actinomycetes. UTTAR PRADESH JOURNAL OF ZOOLOGY, 45(11), 218–226. https://doi.org/10.56557/upjoz/2024/v45i114087 Uttar Pradesh Journal of Zoology Volume 45, Issue 11, Page 218-226, 2024; Article no.UPJOZ.3511 ISSN: 0256-971X (P) Optimization Studies of Anticancer Enzyme -asparaginase from Actinomycetes Reshmi Gopalakrishnan a* and B. Thamarai Selvi a a Department of Microbiology, Sri Ramakrishna College of Arts & Science for Women, Coimbatore-641044, India. Authors’ contributions This work was carried out in collaboration between both authors. Both authors read and approved the final manuscript. Article Information DOI: https://doi.org/10.56557/upjoz/2024/v45i114087 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://prh.mbimph.com/review-history/3511 Received: 07/03/2024 Accepted: 10/05/2024 Published: 13/05/2024 ABSTRACT Discovery of new sources for therapeutic enzymes has much attention in medicine. Although bacterial-derived L-asparaginase is present in the market, studies demonstrated that asparaginase from actinomycetes might be more effective and have fewer side effects. The current study emphasizes the production of asparaginase enzyme from actinomycetes isolated from the Chikmagalur forest, Karnataka, India can be exploited as a possible source for significant production of this enzyme. The physical and chemical parameters were monitored, and maximum yield was obtained at pH 8, temperature at 30 o C in the presence of carbon source(glucose) & nitrogen source(peptone). L-asparaginase, an enzyme with clinical & industrial applications, is a potent molecule with anticancer and antioxidant properties. Keywords: L-asparaginase; actinomycetes; anticancer; antioxidant and asparagine. Original Research Article