Research Article Open Access Biochemistry & Analytical Biochemistry B i o c h e m i s t r y & A n a l y t i c a l B i o c h e m i s t r y ISSN: 2161-1009 Chaturvedi et al., Biochem Anal Biochem 2017, 6:1 DOI: 10.4172/2161-1009.1000306 Volume 6 • Issue 1 • 1000306 Biochem Anal Biochem, an open access journal ISSN: 2161-1009 *Corresponding author: Awanish Kumar, Department of Biotechnology, National Institute of Technology, Raipur – 492010, Chhattisgarh, India, Tel: 8871830586; E-mail: drawanishkr@gmail.com, awanik.bt@nitrr.ac.in Received: October 31, 2016; Accepted: January 21, 2017; Published January 24, 2017 Citation: Chaturvedi AD, Nagarajan K, Pal D, Kumar A (2017) Escherichia coli Generate Oxidative Stress and Enhance Lipid Peroxidation in the Kidney of the Rat. Biochem Anal Biochem 6: 306. doi: 10.4172/2161-1009.1000306 Copyright: © 2016 Chaturvedi AD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract The urinary tract is generally infected by Escherichia coli bacteria. They commonly originate in the patient's own bowel, and infection occurs mostly via the ascending route. In this study, we have observed the influence of E. coli on oxidative stress generation and lipid peroxidation in the kidney of the rat. E. coli were taken from the soil, urine, buffalo intestine and goat intestine for this study. Rats were infected with isolated E. coli from different sources and Lipid peroxidation, glutathione assay was done to achieve the goal of this study. Percentage survival data showed that the E. coli isolated from urine had more lethargy phenomena because their survival present was 66.66% and the mortality rate was higher in this group. Although E. coli isolated from Goat intestine has also shown the same mortality, but the E. coli isolated from urine sample shown this mortality from the second day whereas the E. coli isolated from goat intestine from the third day. By and large data indicated that the E. coli isolated from urine sample generated high oxidative stress and damage rat kidney because the kidney is an organ frequently exposed to oxidative stress. Overall, free radical generated due to E. coli infection further enhances lipid peroxidation in the rat which is harmful to the physiology because it may cause a renal disorder in the rat. Escherichia coli Generate Oxidative Stress and Enhance Lipid Peroxidation in the Kidney of the Rat Amiy Dutt Chaturvedi 1 , Nagarajan K 2 , Dharm Pal 3 and Awanish Kumar 4 * 1 Department of Plant Ecology and Environmental Science, CSIR-National Botanical Research Institute, Lucknow, India 2 Department of Veterinary Pathology, Madras Veterinary College, Chennai, India 3 Department of Chemical Engineering, National Institute of Technology Raipur, India 4 Department of Biotechnology, National Institute of Technology Raipur, India Keywords: E. coli; Virulence; Rat; Kidney; Lipid peroxidation Introduction Our gastro-intestinal tract is a biologically diverse and complicated system which contains numerous bacterial species. Intestinal microbes harbor a diverse microbial community, oſten containing opportunistic bacteria with virulence potential [1]. Both the host and bacteria are thought to derive benefit from each other. While most of the activities of the normal flora benefit their host, some of the normal flora are parasitic (live at the expense of their host), and some are pathogenic (capable of producing disease) [2]. Diseases that are produced by the normal flora in their host are called endogenous diseases. Most endogenous bacteria are opportunistic in infection [1]. Among endogenous bacteria, Escherichia coli are generally harmless for humans but some of them are parasitic and may cause life dangerous complication like a severe kidney damage function or even sepsis. E. coli may live for months in water and ground but they get the opportunity they multiply very quickly and cause digestion problems [3,4]. We can describe E. coli as opportunistic bacteria only in the specific situation when it arrives from the intestine to other organs and tissues and causes illnesses by producing Shiga toxin [5,6]. e most frequent are urinary tract and sexual organs infections. E. coli outbreak has killed many persons in the USA in and other parts of the world [7,8]. Virulence factors of E. coli are studied and reported in different population by many research groups [9-13]. E. coli infection was also reported in domestic animals like rabbits, cats, dogs, goats and horses [14]. e main site of E. coli infection is kidney and their infection is spread through blood [15]. erefore in this study kidney and serum burden was measured aſter total dosages regimen (infection time period) and data of their dilution series showed that kidney and blood burden was higher in buffalo having E. coli infection. e sources of E. coli were soil, urine, buffalo intestine and goat intestine. is study was designed to investigate the antibiotic resistance, serum analysis and virulence of E. coli (isolated from the different source) in the rat. Materials and Methods Concept design of the study e present study was emerged from isolation of E. coli from soil sample, urine sample, goat (G) and buffalo (B) intestine. ey were collected locally and stored at -20°C until use. Isolation of E. coli bacteria Samples were firstly inoculated in 1% tryptone broth for 24 hours for the isolation of E. coli from different sources mentioned above. en the broth was again inoculated on Mac-konkey Agar media for 24 hrs. en the pink colonies were selected and again transferred on EMB agar media for 24 hrs. e metallic shine colonies were selected and stored in LB agar media for at 4°C until use. Biochemical tests for identification of bacteria Urease test: Urea agar medium and urea broth were prepared and sterilized by autoclaving at 121°C. Media was poured into the tubes and allows them to solidify in a slanting position to form slopes. e tubes were labeled with the name of the inoculating organism. Inoculate the liquid and agar media with a transfer loop. Incubate the slants/broths for 24-48 hrs at 37°C. Carbohydrate catabolism by microorganisms test: Glucose