1 FRET and optical trapping reveal mechanisms of actin-activation of the power stroke and phosphate-release in myosin V 1 Laura K. Gunther, 2 John A. Rohde, 1 Wanjian Tang, 1 Joseph A. Cirilo Jr., 3 Christopher P. Marang, 3 Brent D. Scott, 2 David D. Thomas, 3 Edward P. Debold, and 1 *Christopher M. Yengo 1 Department of Cellular and Molecular Physiology, Penn State College of Medicine, Hershey, PA 17033 2 Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455 3 Department of Kinesiology, University of Massachusetts, Amherst, MA 01003 *Corresponding author: Christopher M. Yengo E-mail: cmy11@psu.edu Running Title: Coordinating actin binding and force generation in myosin Keywords: Myosin, actin, ATPase, fluorescence resonance energy transfer (FRET), optical tweezers, single-molecule biophysics https://www.jbc.org/cgi/doi/10.1074/jbc.RA120.015632 The latest version is at JBC Papers in Press. Published on October 14, 2020 as Manuscript RA120.015632 by guest on December 11, 2020 http://www.jbc.org/ Downloaded from