Environmental Sustainability: Concepts, Principles, Evidences and Innovations - ISBN: 978-93-83083-75-6 88 Bioremediation of Monocrotophos and Malathion by Bacillus sp. Bini Samal 1 , Parul Bhatt Kotiyal 2 1 Ecology and Environment Division, Forest Research Institute, Dehradun, Uttarakhand, India ABSTRACT The use of chemical pesticides has brought benefits such as the increment of agricultural production, soil productivity and products quality, which is reflected in economical benefits, vector disease control and in general, in public health. There are some bacteria in the soil which can help in solving the problem of Pesticide Pollution. The isolation of pesticide degrading bacteria was carried out using selective media (Bacillus cereus medium) and the isolated bacterial isolate were identified as Bacillus sp. The growth of the pesticide degrading isolate was assessed in Bushnell haas broth containing different (0.5%, 1%, 1.5%) of Malathion and Monocrotophos. Growth was observed in all the three concentrations of Malathion and Monocrotophos by the bacteria. But the best degradation potentials were observed by Bacillus sp. at 0.5% concentration of pesticides. Colonies of bacillus sp. were seen in minimal medium agar (Bushnell Haas Agar) inoculated with different concentrations of Malathion and Monocrotophos also. The bioremediation potential of bacteria was confirmed by inoculating pesticide and bacteria in chickpea plants (pot experiment). The growth of the plants showed a positive growth when both pesticide and bacteria were inoculated as compared to only pesticide inoculation. Bacillus sp. has bioremediation potential for the degradation on Malathion and Monocrotophos at all the three concentrations (0.5%, 1%, 1.5%). But the maximum values of absorption were obtained at 0.5% concentration. Bacillus sp. has shown better results for Malathion. Keywords: Bacteria, bioremediation, malathion, monocrotophos. 1. INTRODUCTION Bioremediation uses living systems or biological products to biodegrade anthropogenic waste, with the objective being reduction of waste to chemical forms that can be assimilated into natural cycles. It leads to actual reduction and detoxification of wastes, ideally degraded to CO2 and water, when there are organic compounds. The goal of bioremediation is to biotransform toxic materials into nontoxic ones and to make accumulating anthropogenic waste enter natural biogeochemical cycles