Journal of Chemical and Pharmaceutical Research, 2012, 4(2):1441-1448 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 1441 Chemical Investigation of Seed of Ipomoea hederacea and its Biological Activity B. P. Singh* and Sandhya Singh Department of Chemistry, University Institute of Engineering & Technology, C.S.J.M. University, Kanpur, India _____________________________________________________________________________________________ ABSTRACT Ipomoea is the largest genus in the flowering plant family "Convolvulaceae” with over 500 species, most of these are called morning glories. Ipomoea hederacea considered as a valuable herb for indigenous medicine is renowned for its medicinal properties. Ipomoea hederacea is a favored drug for different diseases because of its oral effectiveness, good safety profile availability in India. The seed of this plant have also been used in both analgesic and antiseptic properties. The present work is aim to investigate the phytochemical investigations of different extracts of seed of Ipomoea hederacea. The methanolic extract of seed was directly chromatographed over silica gel column eluted with solvent petroleum ether, chloroform, acetone and methanol to their increasing order of polarity. The various extracts were directly separated by column chromatography and monitored by thin layer chromatography. The extracts were characterized on the basis of various spectral techniques such as IR, 1 H NMR, and mass spectroscopy. The antibacterial study performed against to bacteria species via; Escherichia coli and pseudomonas aeruginosa. The methanol extracts of seed of Ipomoea hederacea exhibited varying level of antibacterial activity, with minimum inhibitory concentration (MIC) of 2 mg/ml against both bacteria. The methanol extract was found to be more active than the other extract like petroleum ether, chloroform and acetone. The antifungal activity of these extracts was also performed against to alternaria brassica, alternaria braceacola, aspergillus Niger fungal strain. The methanol and chloroform extracts shows moderated as well as significant activity against the strains. Keywords: Ipomoea Hederacea, Biological activity, Seed, Herbicides, Diabetes. _____________________________________________________________________________________________ INTRODUCTION India is a home to abundant medicinal plant and animal life and has a wide range of climates that accommodate a diversity of species throughout the country. A large number of medicinal plants are present in hill region which are known but these have not been investigated chemically yet and put to use in modern system of medicine. Herbicides have been effective in reducing the effect of weeds on crop yield for more than 50% [1]. Moreover recommended rates of herbicides are often higher than what is actually needed for effective weed control. There are about 20,000 species of tropical plant in Mexico out of which 5,000 are considered to possess medicinal values, which is yet to be documented and evaluated by means of scientific approaches [2]. Medicinal herb is considered to be a chemical factory as it contains multitude herbs are using in the treatment of heart disease, skin disease, high blood pressure, pain, asthma and other problems. Ipomoea hederacea is commonly known as Ivy leaf morning glory, pharbits seeds and kaladana. The most important species Ipomoea batatas extensively cultivated for its edible root tubers. A number of species have been introduced in India and many species are grown in gardens for ornamental purposes, some are of medicinal value [3,4]. Leaf wax contains primarily a variety of short and long chain hydrocarbons, alcohols, acids, esters, aldehydes and triterpenes. Morning glory leaves are easily removed with defoliants or disicants, but stems or tough and wiry causing them to be more resistant to drying. The seeds were found to have both analgesic and antiseptic properties. Chemists identified indolizidine alkaloids in the seeds, to which the analagesic properties had attributed. Different formulation of the crude drug has been made, the treatment of skin