Jurnal Biodjati 8(2):191–202, November 2023 http://journal.uinsgd.ac.id/index.php/biodjati e-ISSN : 2541-4208 p-ISSN : 2548-1606 Effect of Growth Space on The Productivity of Maize Using Three Sisters Cultivation with Bee Pollination Nadia Damika Putri1, Acep Purqon2, Ramadhani Eka Putra3* INTRODUCTION The increasing number of food needs due to the population growth every year is one factor that encourages agricultural pro- duction. Food security is an essential factor because it is estimated that the population in Indonesia will continue to increase, with an estimated 319 million people in 2045 (Statis- tics Indonesia (BPS), 2020). Thus, the future challenge in agriculture is to provide enough nutrition for the growing human population in the urban area. Urban agriculture may provide a foundation for asustainable food production system and other benefits related to a sustain- able city (Nogeire-McRae et al., 2018). This system can be a solution for cutting the food supply chain and supporting micro-household food independence, which produces food for local needs (Ghosh, 2008; Eigenbrod & Gru- da, 2015). This independent food production can reduce the costs of food purchases, adjust production results to the dietary preferences of each individual, and consume fresher and more affordable food (Andini et al., 2021). However, urban agriculture's common challenges are low land availability and de- Citation Putri, N. D., Purqon, A. & Putra, R. E. (2023). Effect of Growth Space on The Productivity of Maize Using Three Sisters Cultivation with Bee Pollination. Jurnal Biodjati, 8(2), 191–202. DOI: 10.15575/biodjati.v8i2.19227 *Corresponding author Abstract. The increasing number of food needs is one of the driv- ing factors for increasing agricultural production, but there are con- straints on the availability of land. A polyculture system with corn, beans, and pumpkins, commonly known as the three sisters, can create positive interactions that can enhance the growth and development of each plant. This system has a vast potency to be applied to urban farming inside a grow bag because it does not require ample space, the placement of plants is flexible, and it produces a variety of yields. However, it is necessary to assess the effect of growing space on the growth of maize (Zea mays) cultivated by the three-sister system. This study used a completely randomized design with three treatments and six replications. The treatment consists of three planting spaces with various growing bags (treatment A:75 L, B:100 L, and C:200 L). The results of this study showed that the highest corn productivity was in the largest growing space (treatment C), which weighed 318.40 g/cob, and without husks 246.42 g/cob, but not significantly different from treatment B (grow bag 100 L), which weights 316.20 g/cob and with- out pods of 240.63 g/cob. This study found that the 100 L grow bag was the optimal growing space for planting corn in containers using the three sisters technique. Keywords: corn, grow bag, growth space, three sister 1,3 Agricultural Engineering, School of Life Sciences and Technology, Jl. Let. Jend. Purn. Dr. (HC) Mashudi No.1, Jatinangor, Sumedang, West Java- Indonesia, 45363, 2Department of Physic, Faculty of Mathematics and Natural Sciences, Jl. Ganesha No. 10 Bandung, West Java, Indonesia, 45363 e-mail: 1 elnadiadamikaputri@gmail.com 2 acep.purqon@gmail.com 3* ramadhaniputra@gmail.com Received: June 19, 2023 Revise from: June 27, 2023 Accepted: August 08, 2023 Copyright © 2023 Jurnal Biodjati This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/)