Dangi et al Journal of Drug Delivery & Therapeutics; 2014, 4(2), 182-185 182 © 2011, JDDT. All Rights Reserved ISSN: 2250-1177 CODEN (USA): JDDTA Available online at http://jddtonline.info RESEARCH ARTICLE SYNTHESIS, ANTICONVULSANT ACTIVITY OF SOME NOVEL BENZIMIDAZOLE ACETOHYDRAZIDES Girija Dangi 1* Neeraj Kumar 1 , Chandra Shekhar Sharma 2 , Lalit Singh Chauhan 3 1 Department of Pharmaceutical Chemistry, Geetanjali Institute of Pharmacy, Udaipur, India 2 Department of Pharmaceutical Chemistry, BN College of Pharmacy, Udaipur, India 3 Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur INTRODUCTION: Benzimidazole is very useful heterocyclic compound/intermediate for the development of molecules of pharmaceutical or biological interest. 1 Derivatives of benzimidazole possess a wide range of pharmacological activities such as Anti-neoplastic, analgesic, Anthelmintics, Anti-ulcer, anti-inflammatory, Antifungal, Anti-histaminic, Antiviral and Anti- diabetic. 2-7 Numerous publication have shown that benzimidazole derivatives also have anticonvulsant activity. 8-12 An attempt has been made in our study to synthesize Benzimidazole acetohydrazide derivatives to get as potent anticounvulsant compounds. The newly synthesized Benzimidazole acetohydrazide derivatives were evaluated for anticonvulsant activity by the MES method using phenytoin as standard. MATERIAL AND METHODS: 1H-benzimidazole (1a). A mixture of o- phenylenediamine (27 g, 0.25 mol) and formic acid (16 ml, 85 %) was refluxed on a water bath for 2 h. The reaction mixture was cooled and its pH was adjusted to 8 by addition of sodium hydroxide (10 %) solution. The crude product so obtained was filtered, thoroughly washed with ice cold water and dried. The dried product was boiled with decolourising charcoal (2 g) in water (400 ml) for 15 min and filtered while hot. The solution thus obtained was allowed to cool at 10 ° C for 30 min, filtered and dried. The completion of reaction was monitored by running TLC. Yield: 88 %, R f value: 0.46, M.p. 170-172 ºC. IR (KBr in cm -1 ): 3115 (N-H), 3048 (Ar. C-H), 1362 (C=N). Anal. calcd. for C 7 H 6 N 2 (118.14): C, 71.17; H, 5.12; N, 23.71. Found: C, 71.22; H, 5.16; N, 23.31 %. LCMS m/z: 118.1 (M+). NH 2 NH 2 RCOOH NH N R -HCl N N R NH NH . H O N N R CH 2 COOC 2 H 5 (1a-1f) (2a-2f) (3a-3f) CH 2 CONHNH 2 Comp. R 3a -H 3b -CH 3 3c -CH 2 C 6 H 5 3d -C 6 H 5 3e 4-ClC 6 H 4 3f 4-(CH 3 )NC 6 H 4 ClCH 2 COOC 2 H 5 Scheme 1 Reaction Protocol for the synthesis of 3a-3f *Corresponding Author: Girija Dangi Department of Pharmaceutical Chemistry, Geetanjali Institute of Pharmacy, Udaipur, India Email: girijadangi@gmail.com ABSTRACT: A series of some novel benzimidazole acetohydrazide derivatives was synthesized and characterized by TLC, MP, elemental analysis, IR, Mass and 1 H NMR spectroscopy. These compounds were screened for anticonvulsant activities by Maximum Electroshock seizure model in male wistar rats and compared with the standard drug phenytoin. Keywords: Benzimidazole, hydrazide, Anticonvulsant, MES