Abstract: Coffee silver skin (CSS), a by-product of the coffee industry, is derived from the seed coat during the roasting of green coffee beans. Owing to its high content of phenolic compounds, antioxidants, dietary fibre, and prebiotic properties, CSS has gained interest as a functional food ingredient. This study aimed to evaluate the potential of CSS as a major component in a smoothie formulation, focusing on its antioxidant capacity (DPPH assay), cytotoxicity, effects on human dermal fibroblasts (HDF), microbial stability, and shelf-life. CSS extract was obtained using the cold maceration technique and assessed for antioxidant and cytotoxic activities. It was subsequently incorporated into smoothies at varying concentrations (1g, 2.5g, 3g, 3.5g, and 5g), followed by sensory evaluation, and monitoring of microbial activity and shelf-life at seven-day intervals. Nutritional analysis revealed that CSS is rich in dietary fibre (50%-60%), protein (16%- 19%), and fats (1.56%-3.28%), underscoring its value as a functional food. The extract exhibited no cytotoxic effects up to a concentration of 1 g/ml. Incorporation of CSS into smoothies not only enhanced their nutritional and functional properties but also maintained product stability, supporting its application as a health-promoting beverage ingredient. Keywords: Antioxidant, Coffee silver skin, Cytotoxicity, Health, Microbial activity, Smoothie. International Journal of Biological Innovations http://ijbi.org.in | http://www.gesa.org.in/journals.php https://doi.org/10.46505/IJBI.2025.7202 IJBI 7(2): 110-121 (2025) E-ISSN: 2582-1032 INCORPORATION OF COFFEE SILVER SKIN FOR DEVELOPMENT OF A SMOOTHIE AND A NUTRACEUTICAL PRODUCT Poornima Tiwari and Bhakti A. Mhatre* School of Biotechnology and Bioinformatics D.Y. Patil Deemed to be University, CBD Belapur, Navi Mumbai (M.S.), India *Corresponding author: bhaktiamhatre@gmail.com Article Info: Research Article Received 31.12.2024 Reviewed 31.05.2025 Accepted 01.07.2025 GESA GLOCAL ENVIRONMENT & SOCIAL ASSOCIATION Cite this article as: Tiwari P. and Mhatre B.A. (2025). Incorporation of coffee silver skin for development of a smoothie and a nutraceutical product. International Journal of Biological Innovations. 7(2): 110-121. https://doi.org/10.46505/IJBI.2025.7202 This is an Open Access Article licensed under a Creative Commons license: Attribution 4.0 International (CC-BY). It allows unrestricted use of articles in any medium, reproduction and distribution by providing adequate credit to the author (s) and the source of publication. physical and emotional health, offering protection against various health conditions such as heart disease, obesity, cardiovascular diseases, and type 2 diabetes (Klingel ., 2020). et al A healthy diet emphasizes the reduction of salt, sugar, and processed food intake. As such, the concept of 'eating smart' should be prioritized to maintain optimal health. In modern times, the INTRODUCTION Health and maintaining a healthy lifestyle are critically important due to their profound impact on overall human well-being. A healthy lifestyle is typically associated with a fit body and an active, refreshed mind (Jannissen and Huynh, 2018). One of the fundamental components of such a lifestyle is a balanced and nutritious diet. Eating well contributes significantly to both