Research Article
EffectofaCombinationof Rosa canina FruitsandAppleCider
Vinegar against Hydrogen Peroxide-Induced Toxicity in
Experimental Animal Models
Driss Ousaaid ,HassanLaaroussi ,MeryemBakour ,AsmaeElGhouizi ,
NaoualElMenyiy,BadiaaLyoussi ,andIlhamElArabi
Laboratory of Natural Substances, Pharmacology Environment, Modeling,Health and Quality of Life,
Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, Morocco
Correspondence should be addressed to Driss Ousaaid; driss.ousaaid@usmba.ac.ma
Received 24 April 2021; Revised 16 December 2021; Accepted 23 December 2021; Published 5 January 2022
Academic Editor: Constantin Apetrei
Copyright©2022DrissOusaaidetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Oxidative stress is the trigger of several diseases. It is an imbalance between the production of free radicals and antioxidants. is
studyaimstoevaluatetheantioxidantcapacityandtheprotectivepropertyof Rosacanina fruitsandapplecidervinegarcombined
or not against hydrogen peroxide (H
2
O
2
)-induced toxicity in Wistar rats. e experiment included five groups: group 1 received
distilled water (10mL/kg b.wt), group 2 received H
2
O
2
10% (10mL/kg b.wt), group 3 received H
2
O
2
10% (10mL/kg b.wt) and
applevinegar(2mL/kgb.wt);group4receivedH
2
O
2
10%(10mL/kgb.wt)andapplevinegarsupplementedwith Rosacanina fruits
extract (300mg/kg b.wt); group 5 received H
2
O
2
10% (10mL/kg b.wt) and extract of Rosa canina fruits (300mg/kg b.wt). e
dosesweregivenoncedailyviaagavage.eantioxidantcapacityofapplevinegarand Rosacanina extractwasanalyzed,andAST,
ALT, PAL, urea, and creatinine were determined on day 22 of the experiment. In addition, the kidney and the liver tissues were
analyzed. e results showed that H
2
O
2
caused a significant elevation of blood urea, blood creatinine, and transaminases. e
histopathology examination revealed that H
2
O
2
caused congestion, hemorrhage, and Bowman’s space enlarged. On the other
hand,theresultsclearlyshowedthatapplevinegarand Rosacanina fruitscounterbalancethebiochemicalandhistologicalchanges
inducedbyH
2
O
2
. In conclusion, the two natural products studied in this work are effective against the harmful effect of oxidative
stress, which explains their use in traditional medicine.
1.Introduction
Oxidative stress induces an increase in the production of free
radicals considered as drivers of different pathogenesis and
organ damages. It could disturb the antioxidant system and
lead to direct cellular injury stress in the liver and kidney [1].
Numerous factors can induce this imbalance such as acidosis,
nitric oxide, transition metals, LDL oxidation [2], and toxic
agentsincludinghydrogenperoxide(H
2
O
2
)[3].Furthermore,
H
2
O
2
can permeate across the membrane as a destructive
agent and generate hydroxyl radicals [3]. Other producers of
reactive oxygen species (ROS) can induce necrosis and ap-
optosis [4]. Numerous reports have well established that
oxidative stress is the major cause of various pathologies.
Nowadays, the use of natural products turns out to be a
goodalternativetochemicals,thesideeffectsofwhichcanbe
serious [5, 6]. Fruits and their byproducts have tremendous
beneficial properties. Among a long list of fruits wealthy on
bioactive compounds with enormous utilizations in folk
medicine, we found Rosa canina fruits. Several studies
evoked that Rosa Canina fruits were used in alternative
medicine to treat different diseases including diabetes
mellitus, colds, fever, scurvy, and kidney diseases [7]. e
wealth of hips on phytochemicals with high antioxidant
ability improves cell longevity and obstructs skin aging as
previously proved by Phetcharat et al [8]. It has been
documentedthathipsextractcanbeusefulintheprevention
of histological damages, oxidative stress, and functional
Hindawi
Journal of Food Quality
Volume 2022, Article ID 7381378, 9 pages
https://doi.org/10.1155/2022/7381378