Int. J. Adv. Sci. Eng. Vol.11 No.4 4679-4702 (2025) 4679 E-ISSN: 2349 5359; P-ISSN: 2454-9967 Mariappan Srilatha et al., International Journal of Advanced Science and Engineering www.mahendrapublications.com ABSTRACT: The present study aims to assess antioxidant and thrombolytic efficacy of Curcuma amada Roxb. (Mango ginger) rhizome extracts terpenoid and phenolic compounds as new therapeutic prospects to serve as fibrinolytic agents. Curcuma amada extract was fractioned with different solvents based on polarity by using pure methanol, ethanol, hydro ethanol, and water. Preliminary phytochemical analysis of all four Curcuma amada extracts revealed that hydroethanolic extract rich in phenol and terpenoid phytocompounds, so hydroethanolic extract alone took for further analysis. Quantitative phytochemical analysis of hydroethanolic extract of Curcuma amada reveals that rich in alkaloid (58±1.08 mg AE/g) content than flavonoid and protein (44±0.78 QE mg/g and 37±0.72 mg/g) contents respectively. Further in vitro thrombolytic analysis reveals hydroethanolic extract of Curcuma amada exhibited the highest and most significant clot lysis (42.35%) of human blood compared to standard streptokinase (29.47%). Hydro ethanolic extract of Curcuma amada rhizome showed seven major compounds peaks in GCMS analysis such as geraniol, curcumin, camphene, eucalyptol, carvacrol, turmerone, nortrachelogenin belongs to terpenoid, phenolic and lignan group of phytocompounds. Further, In silico molecular docking analysis of seven identified compounds from rhizome shown compounds curcumin (-7.8, -7.8 kcal/mol) and nortrachelogenin (-7.1, -6.9 kcal/mol) excellent binding affinity with thrombolytic target proteins tissue plasminogen activator (PDB 1A5H) and coagulation factor XA (PDB 1NFY). Finally molecular dynamic simulation studies shown curcumin possess excellent simulation trajectory with tissue plasminogen activator (PDB 1A5H) and coagulation factor XA target protein than with standard drug colchicine. Further clinical study is needed to understand the exact thrombolytic mechanism of Curcuma amada rhizome. KEYWORDS: Antioxidant, Curcuma amada, Thrombolytic, Curcumin, In silico, docking https://doi.org/10.29294/IJASE.11.4.2025.4679-4702 ©2025 Mahendrapublications.com, All rights reserved *Corresponding Authors: tmalaisamy@gmail.com/sriramkarthik83@gmail.com Received: 11.03.2025 Accepted: 20.04.2025 Published on: 23.06.2025 Exploration and Validation of Thrombolytic Phytocompounds from Curcuma amada Roxb through In Vitro and In Silico Approaches Mariappan Srilatha 1 , Vincent Aroulmoji 2 , Thirumalaisamy Rathinavel 1 *, C.Roumana 3 , Mohan Hariharan 4 , Meganathan Bhuvaneswari 1 , Subramanian Ammashi 5 , Ramalingam Karthik Raja 4 * 1 Department of Biotechnology, Sona College of Arts and Science, Salem (Dt.) – 636 005, Tamil Nadu, India 2 Centre for Research & Development, Mahendra Engineering College (Autonomous), Mallasamudram, Namakkal (Dt.) - 637 503, Tamil Nadu, India 3 PG and Research Department of Physics, Government Arts College (Autonomous), Salem (Dt.) -636 007, Tamil Nadu, India 4 Center for Applied Research, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai – 602 105, Tamil Nadu, India 5 Department of Biochemistry, Bharathi Womens College (Autonomous), Affiliated to Madras University, Chennai -600 108, Tamil Nadu, India INTRODUCTION According to the World Health Organization, Cardiovascular disease (CVD) encompasses conditions affecting the heart and blood arteries, including heart disease, cerebrovascular disease, and other dysfunctions. An estimated 17.5 million people, or 31% of all deaths globally, are thought to be related to cardiovascular diseases (CVDs) [1,2]. Furthermore, the majority of deaths from cardiovascular diseases (CVDs) are caused by strokes and heart attacks. More