https://www.alliedacademies.org/journal-biochemistry-biotechnology/ Short Communication J Biochem Biotech 2021 Volume 4 Issue 4 Materials and Methods Collection of plant material The plants A. viridis was collected from open fields of Lalitpur district of Nepal. Leaves and seeds of the plants were separated and washed properly with distilled water to remove any impurities and dust settled on the surface of these plant parts. All Plant parts were dried under shade at room temperature for the prevention of loss of any active constituents. The dried leaves and seeds of the plants were grounded separately to coarse powder using mechanical blenders followed by fine extraction using organic solvents. Plant extraction: All plant extracts were prepared using the soxhlet extraction method. 20 grams of dry powder from leaves, seed and whole plant of A. viridis were extracted with 200 ml of methanol separately in soxhlet extractor until the final extraction was colorless. These methanol extracts were then subjected to rotary evaporator at 64.7ºC respectively. The crude extracts obtained were stored at 4ºC in airtight glass bottles for further use. Preliminary phytochemical screening: The methanol extract of A.viridis was screened for the presence of various phytoconstituents like flavonoids, saponins, glycosides, terpenoids amino acids, alkaloids, carbohydrates, phenolic compounds proteins etc [10,11]. 22 In vitro phytochemical screening, antioxidant potential and in vivo hepatoprotective and renal protective activity of Amaranthus viridis. Ranjit Thakur * , Rakhi Das, Bijay Kumar Das Department of Biochemistry, Universal Engineering and Science College, Chakupat, Lalitpur, Nepal Introduction Amaranthus virdis (Family: Amarantheceae) is distributed in the warmer parts of the world. The amaranthus represents a horticulturally important group of annual herbaceous plants represtented by total of 60 species in the world flora under Amaranthus L., which is the fifth largest genus of the family Amarantheceae. Green amaranthus serve as one of the most delicious leafy vegetables and are rich sources of protein (upto 5.6% on fresh weight basis), requisite vitamins (A,B,C, Folic acid), minerals (Ca, Mg, K, P, Na, N, Fe, Mn, Zn) and fibres (5.25%) [1,2]. Besides, they contain other biologically pro-health compounds such as the antioxidant, squalene, and carotenoids [3] and thus, are recommended as a nutritious food with medicinal properties for young children, lactating mothers and for patients with asthma, hemorrhage, anaemia or kidney complaints. In addition, the whole plant possesses analgesic and anti-pyretic properties and is used for the treatment of pain and fever respectively in traditional system of medicine [4]. Leaves are directly used to eczema, psoriasis and rashes etc., [5] furthermore, the plant possesses anti-proliferative and anti-fungal lactin properties as well as ribosomes inactivating protein beta- carotene [6,7] and anti-viral activities [8]. However, there is not enough scientific reports to support these supposed analgesic and anti-pyretic activities, this has initiated step to conduct the studies to ascertain the authenticity of these important claims of traditional potency [9]. Abstract The aim of the present study was to evaluate the antioxidant activity, phytochemical screening, hepatoprotective and renal protective activity of Amaranthus viridis. The leaf, seed and whole plant extracts of these plant were prepared in pure solvent methanol. The Phytochemical tests were performed qualitatively while the antioxidant activity was determined by 2,2-Di-Phenyl- 1-Picrylhydazyl-Hydrate (DPPH) method and the hepato and renal tests were carried out by enzymatic kits method. The study revealed that the methanol extract of seeds of Amaranthus viridis has shown effective antioxidant activity in DPPH assay technique. The study also showed liver and renal protective activity against paracetamol induced liver damage and Aspirin induced renal damage. Accepted on July 15, 2021 Keywords: Phytochemical screening, Antioxidant activity, Hepato, Renal protective.