The Journal of Middle East and North Africa Sciences 2023; 9(04) http://www.jomenas.org 1 Green Synthesis, Characterization and Anti-Helmintic (Worms) Potency of Copper (II) Oxide and Zinc (II) Oxide Nanoparticles Derived From Azadirachta Indica (Neem) Bark Gongden J. J. 1 • Musa S. 1 • Lohdip Y. N. 1 • Lungaka A. P. 2 • Chollom A. M. 1 • Idongesit N. A. 1 1 Department of Chemistry, University of Jos, Plateau State, Nigeria 2 Department of Chemistry, Plateau State University Bokkos, Nigeria gongdenjj@gmail.com Abstract. Zinc (II) and copper (II) oxide nanoparticles (ZnO-Nps and CuO-Nps) were synthesized from Azadirachta indica (Neem) bark. The complexes were characterized, and their anti-helminths (worms) activities were studied. Direct synthesis was used for the synthesis and characterization using FTIR. UV-visible, XRD and SEM. In-vitro and In-vivo were used to test the biological activities (anti-helminthic). The UV-Visible absorption spectrum of zinc oxide nanoparticles shows the characteristic surface plasma resonance with a wavelength of approximately 200 – 440 nm and a peak maximum at 380 nm with attributes to the formation of nanoparticles. FT-IR Spectroscopy was used to identify the possible biomolecules responsible for reducing zinc nitrate ions and copper sulfate and capping the reduction of zinc nanoparticles. Copper nanoparticles synthesis used Azadirachta indica (Neem) bark extract and the result shows zinc oxide nanoparticles gave the IR bands 3220.4 cm-1, 2102.2 cm-1, 1990.4 cm-1, 1595.3 cm-1, 1401.5 cm-1, 1088.4 cm-1, 771.8 cm-1, 849.8 cm-1, 1028.7 cm-1,. The results observed from copper oxide nanoparticles are 3252.61 cm-1, 2918.62cm-1, 2850.90 cm-1, 2168.92 cm-1, 1636.30 cm-1, 1239.42 cm-1, 1083.80 cm-1, and 1035.91 cm-1. The bands which appeared at 3252.61cm-1 and 2918.62cm- 1 corresponds to O-H stretching and symmetric C.H. stretching respectively. XRD shows sharp peaks of the nanoparticles, which indicates the formation of nanoparticles and crystallinity. SEM analysis also showed a magnified image of the nanoparticles, further confirming their formation. The nanoparticles were found to be potent in anti-helminthic activities at 200mg/ kg. To cite this article [Gongden J. J., Musa S., Lohdip Y. N., Lungaka A. P., Chollom A. M. & Idongesit N. A. (2023). Green Synthesis, Characterization and Anti-Helmintic (Worms) Potency of Copper (II) Oxide And Zinc (II) Oxide Nanoparticles Derived From Azadirachta Indica (Neem) Bark. The Journal of Middle East and North Africa Sciences, 9(04), 1-6]. (P-ISSN 2412- 9763) - (e-ISSN 2412-8937). www.jomenas.org. 1 Keywords: Nanoparticles; Anti-Helminthes; Characterization; ZnO-Nps; CuO-Nps; Invivo; Invitro; Synthesis. 1. Introduction The field of nanotechnology is one of the most active areas of research in modern materials science. New applications of nanoparticles (N.P.s) and nanomaterials are emerging rapidly. Green synthesis provides advancement over chemical and physical methods as it is cost-effective, environment-friendly, and easily scaled up for large-scale synthesis. Furthermore, there is no need to use high pressure, energy, temperature, and toxic chemicals. Synthesis of N.P.s using biological entities has generated significant interest due to their unusual optical, chemical, photo-electron-chemical, and electronic properties (Mohanpuria et al., 2008). The particles of size less than 100 nm in diameter were not uncommon on the planet earth as these were found in nature due to photochemical volcanic activity, combustion and food cooking, and vehicle exhausts. Nanoparticle synthesis has led to the introduction of nanotechnology in the last two decades which produced novel compounds that are applied in various fields. The pure metals formed in nanoparticles are applied in the field of diagnostics, antimicrobial agents, anti-worm activities, drug delivery, textiles (clothing), electronics, bio-sensing, food industry, paints, cosmetics, medical devices, and treatment of several acute and chronic diseases, such as malaria, hepatitis, and cancer. Different metals are used in nanoparticle synthesis, but zinc oxide and copper oxide are preferred in this study because of their non-hazardous nature. Zinc nitrate is an inorganic compound with the formula ZnO. ZnO is a white powder that is insoluble in water and is widely used as an additive in numerous materials and products, including rubbers, plastics, ceramics, glass, cement, lubricants, paints, ointments, adhesives, sealants, pigments, foods, batteries, ferrites, fire retardants, and first-aid tapes (Hernandezbattez et al., 2008). Although it occurs naturally as the mineral zincite, most zinc oxide is produced synthetically (Marcel et al., 2006). ZnO is a wide-band gap semiconductor of the II-VI semiconductor groups. The