Journal Pre-proof The Beneficial Impact of Silymarin-Loaded Chitosan Nanoparticles on the Quality of Frozen-Thawed Buffalo Sperm: Antioxidant and Anti-Apoptotic Effects. Ali Ali El-Raghi, Ekramy Elmorsy, Mahmoud A.E. Hassan, Walaa M. Essawi, Sara H.M. Hassab, Eman M. Embaby, Basma H. Marghani PII: S0093-691X(25)00151-7 DOI: https://doi.org/10.1016/j.theriogenology.2025.117425 Reference: THE 117425 To appear in: Theriogenology Received Date: 11 February 2025 Revised Date: 2 April 2025 Accepted Date: 3 April 2025 Please cite this article as: El-Raghi AA, Elmorsy E, Hassan MAE, Essawi WM, Hassab SHM, Embaby EM, Marghani BH, The Beneficial Impact of Silymarin-Loaded Chitosan Nanoparticles on the Quality of Frozen-Thawed Buffalo Sperm: Antioxidant and Anti-Apoptotic Effects., Theriogenology, https:// doi.org/10.1016/j.theriogenology.2025.117425. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2025 Published by Elsevier Inc.