How to Cite: Maiti, R. (2022). Hypoglycemic and antioxidant potency of ethyl acetate fraction of hydro-methanolic extract (60:40) of Tamarindus indica Linn. seed in streptozotocin-induced diabetic experimental animal. International Journal of Health Sciences, 6(S5), 11164–11182. https://doi.org/10.53730/ijhs.v6nS5.10964 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 9 March 2022, Manuscript revised: 18 May 2022, Accepted for publication: 27 June 2022 11164 Hypoglycemic and antioxidant potency of ethyl acetate fraction of hydro-methanolic extract (60:40) of Tamarindus indica Linn. seed in streptozotocin- induced diabetic experimental animal Rajkumar Maiti Assistant Professor, Department of Physiology, Bankura Christian College, Bankura - 722 101, West Bengal, India Corresponding author email: rajkumarbcc1976@gmail.com Abstract---Diabetes mellitus is a chronic metabolic syndrome characterized by hyperglycemia due to defective insulin secretion, insulin action or both. Nowadays massive developments in variety of synthetic modern medicines, alternative and traditional medicinal plants are still widely used for the management of diabetes mellitus. In this context when ethyl acetate fraction at the dose of 100 mg body weight/kg body weight was administered for 28 days, significant diminution in the STZ-induced hyperglycaemia was noticed. Plasma insulin and C-peptide levels as well as activities of antioxidant defense enzymes such as catalase (CAT), peroxidase (Px) and superoxide dismutase (SOD) in the hepatic tissue were found to be decreased in diabetic animals. This was corrected after the treatment of ethyl acetate fraction of hydro-methanolic extract of T. indica. Oral glucose tolerance test (OGTT) reveals that the fraction at the above said dose showed a remarkable decrease of blood glucose level in normal and diabetic rat and also increased glucose tolerance. On the other hand, in diabetic animals body weight decreases along with a decrease in total hemoglobin content in blood and increase in HbA1C level. These animals, however, recover towards the control level by means of a fraction treatment. Histopathology of pancreas was performed after ethyl acetate fraction treatment to diabetic rat and the results were compared with the rats having controlled as well as diabetic groups. To evaluate the free radical scavenging activities of ethyl acetate fraction in-vitro study model with ABTS [2,2´-azinobis-(3 ethyl enzothiazoline-6-sulfonic acid)] and DPPH [1,1-diphenyl-2-pycryl hydrazil] were performed along with measurement of IC 50 values 0.027±0.003 and 0.021±0.002 mg/ml respectively in respect of established antioxidant namely butylated hydroxytoluene (BHT). Phytochemical screening showed that constituents like flavonoids, alkaloids, terpenoids and steroids were found in aforesaid fraction