Indonesian Journal of Health Science Volume 5 No.1, 2025 Page | 22 DAUN GANDARUSA (Justicia gendarussa Burm. F) SEBAGAI ALTERNATIF ANTIBIOTIK: STUDI POTENSI FRAKSI AKTIF TERHADAP Staphylococcus aureus Dede Komarudin 1)* , Yulis Adriana 2) , Siva Fauziah 3) , Anissa Mulyani Putri 4) 1) dede.komarudin44@gmail.com, Institut Sains dan Teknologi Al-Kamal 2) yulisadriana@yahoo.com, Institut Sains dan Teknologi Al-Kamal 3) sivafauziahmfarm@gmail.com, Institut Sains dan Teknologi Al-Kamal 4) inisa43@gmail.com, Institut Sains dan Teknologi Al-Kamal * penulis korespondensi Abstract Background: The gandarussa plant (Justicia gendarussa Burm. F.) is known to have flavonoids, alkaloids, triterponoids, tannins, justicin, steroids, and gendarusin A and B. Empirically, the plant can be used as a remedy for bruises, swelling, lumbago, joint pain, fever, antibacterial, ulcers, ulcers, and fractures. Empirically, this plant can be used as a medicine for bruises, swelling, lumbago, joint pain, fever, antibacterial, ulcers, ulcers, and fractures. Objective: This study measured the zone of inhibition of Staphylococcus aureus bacteria of taro leaf extract to determine the antibacterial properties of ethanol extract of gandarusa leaves. Methods: The study tested the inhibition zone by well diffusion method on ethanol extract, n-hexane fraction and ethyl acetate fraction. Ciprofloxacin as positive control and ethanol as negative control. Concentrations of 75; 50; 25; 10; 5; 2.5 were used for control, extract and fraction. Descriptive data using qualitative and quantitative data. Results: The average value of the diameter of the inhibition zone of ciprofloxacin (positive control) has a very strong bacterial inhibition zone, 25.79 mm. While the diameter value of the inhibition zone against the concentration of ethanol extract, n-hexane fraction & ethyl acetate fraction respectively at 75% concentration (25.18 mm; 30.02 mm & 25.36 mm), 50% concentration (22.1 mm; 26.62 mm & 25. 44 mm), 25% concentration (19.22 mm; 23.8 mm & 21.18 mm), 10% concentration (18.27 mm; 16.95 mm & 17.75 mm), 5% concentration (13.36 mm; 12.31 mm & 14.43 mm) and 2.5% concentration (6.1 mm; 12.73 mm & 12.5 mm). Conclusion: Ciprofloxacin 25 μg/ml (positive control) has a zone of inhibition on Staphylococcus aureus bacteria with an average value of 25.79 mm which is included in the very strong category. The highest average value of ethanol extract of gandarusa leaves (Justicia gendarusa Burm. F), n-hexane fraction and ethyl acetate fraction had inhibition on Staphylococcus aureus bacteria respectively 25.18 mm; 30.02 mm and 25.36 mm which is included in the very strong category. Extracts and active fractions from gandarusa leaves (Justicia gendarusa Burm. F) can be used as an alternative inhibition on Staphylococcus aureus bacteria. Keywords: Antibacterial, Gandarusa Leaf, Staphylococcus aureus Abstrak Latar Belakang: Tanaman gandarussa (Justicia gendarussa Burm. F.) diketahui memiliki flavonoid, alkaloid, triterponoid, tannin, justicin, steroid, dan gendarusin A dan B. Secara empiris, tanaman ini dapat digunakan sebagai obat untuk memar, bengkak, sakit pinggang, nyeri sendi, demam, antibakteri, bisul, borok, dan patah tulang. Tujuan: Studi ini mengukur zona hambat bakteri Staphylococcus aureus ekstrak daun talas untuk mengetahui sifat antibakteri ekstrak etanol daun gandarusa. Metode: Penelitian uji daya hambat dengan metode difusi sumuran pada ekstrak etanol, fraksi n-heksana dan fraksi etil asetat. Ciprofloxacin menjadi kontrol positif dan etanol sebagai kontrol negatif. Konsentrasi 75; 50; 25;10; 5; 2.5 digunakan untuk control, ekstrak dan fraksi. Data deskriptif menggunakan data kualitatif dan kuantitatif . Hasil : Nilai rata-rata diameter zona hambat ciprofloxacin (kontrol positif) memiliki zona daya hambat bakteri yang sangat kuat, 25,79 mm. Sedangkan nilai diameter zona hambat terhadap konsentraksi ekstrak etanol, fraksi n-heksana & fraksi etil asetat berturut-turut pada konsentrasi 75% ( 25,18 mm; 30,02 mm & 25,36 mm), konsentrasi 50% (22,1 mm; 26,62 mm & 25.44 mm), konsentrasi 25% (19,22 mm; 23,8 mm & 21,18 mm), konsentrasi 10% ( 18,27 mm; 16,95 mm & 17.75 mm), konsentrasi 5% (13,36 mm; 12,31 mm & 14,43 mm) dan konsentrasi 2.5% (6,1 mm; 12,73 mm & 12,5 mm). Kesimpulan: Ciprofloxacin 25 μg/ml (kontrol positif) memiliki zona daya hambat pada bakteri Staphylococcus aureus dengan rata-rata nilai 25,79 mm yang termasuk kedalam kategori sangat kuat. Rata-rata nilai tertinggi ekstrak etanol daun gandarusa (Justicia gendarusa Burm. F), fraksi n-heksan dan fraksi etil asetat memiliki daya hambat pada bakteri Staphylococcus aureus berturut-turut sebesar 25,18 mm; 30,02 mm dan 25,36 mm yang termasuk kedalam kategori sangat kuat. Ekstrak dan fraksi aktif dari daun gandarusa (Justicia gendarusa Burm. F) dapat digunakan sebagai alternatif penghambatan pada bakteri Staphylococcus aureus. Kata kunci: Antibakteri, Daun Gandarusa, Staphylococcus aureus