Jurnal Media Analis Kesehatan ISSN : 2087-1333 (Print) ISSN : 2621-9557 (Online) 179 Vol. 14 No. 2 Desember 2023 DOI : https://doi.org/10.32382/jmak.v14i2.251 POTENSI KULIT SINGKONG SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Aspergillus niger DAN Rhizopus oryzae DENGAN MODIFIKASI KONSENTRASI Potential of Cassava Peel as an Alternative Growth Media of Aspergillus niger and Rhizopus oryzae with Concentration Modification Artati 1 , Risky 1 , Rafika 1 , Zulfian Armah 1 , Syahida Djasang 1 , Asriyani Ridwan 2 , Aan Yulianingsih Anwar 3 1 Prodi Sarjana Terapan Teknologi Laboratorium Medis, Poltekkes Kemenkes Makassar 2 Stikes Panrita Husada 3 Poltekkes Kemenkes Ternate E-mail korespondensi: artati@poltekkes-mks.ac.id / 085101429407 ABSTRACT Waste is the waste product of a production process or materials that are no longer valuable or worthless, including waste from food processing processes. One of the main food wastes produced in developing countries is cassava peel from a plant with the Latin name Manihot ultisima. Cassava skin is the outer layer of the tuber which functions as a protector for the cassava flesh. This research aims to prove that cassava peel can be used as raw material for making alternative media for fungal growth and to find out the most effective concentration to use. This research is experimental using cassava skin as a sample. In this research, data analysis was carried out using the One-Way Anova Test. Concentrations of 5%, 10%, 15%, 20%, 25% and 30% were made as alternative media for the growth of Aspergillus niger and Rhizopus oryzae fungi and SDA media as a control. During the incubation period for these two species, both Aspergillus niger and Rhizopus oryzae experienced growth which became bigger day by day. The conclusion of this research is that cassava peel can be used as a natural ingredient that can be used as an alternative medium for fungal growth. In the alternative media for cassava peel, the most effective concentration of the Aspergillus niger species is 20%, while for the Rhizopus oryzae species it is 15%. Keywords: Alternative Media, Cassava Peel, Concentration ABSTRAK Limbah adalah hasil buangan dari sebuah proses produksi atau bahan yang tidak lagi bernilai dan tidak berharga, termasuk limbah proses pengolahan makanan. Limbah makanan utama yang dihasilkan di negara-negara berkembang salah satunya adalah kulit singkong dari tanaman dengan nama latin Manihot ultisima. Kulit singkong merupakan lapisan terluar dari umbi yang berfungsi sebagai pelindung bagi daging singkong. Penelitian ini bertujuan untuk membuktikan kulit singkong dapat dijadikan sebagai bahan baku pembuatan media alternative bagi pertumbuhan jamur serta mengetahui konsentrasi