Vol. 10 No. 1 January–April 2022 Available online at IJTID Website: https://e-journal.unair.ac.id/IJTID/ Original Article The Longevity of Aedes aegypti Larvae in Several Water Sources in Surabaya Antonio Ayrton Widiastara 1 , Gabriel Pedro Mudjianto 1 , Etik Ainun Rohmah 2 , Hengki Anggara Putra 3 , Martha Indah Widia Ningtyas 3 , Sri Wijayanti Sulistyawati 4,5 , Suhintam Pusarawati ,4,5 , Fitriah 5 , Kasiyama Desi Indriyani 6 , Alpha Fardah Athiyyah 7 , Sukmawati Basuki 4,5* 1 Medicine Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 2 Entomology Study Group/Laboratory of Entomology, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia 3 Master Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 4 Department of Medical Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 5 Malaria Study Group/Laboratory of Malaria, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia 6 Laboratory of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 7 Department of Child Health, Dr. Soetomo Hospital/Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Received: 16 th December 2021; Revised: 2 nd March 2022; Accepted: 8 th March 2022 ABSTRACT Aedes aegypti transmits the dengue virus that causes Dengue Viring the high number of DVI cases is the existing breeding places of Ae. aegypti. The water sources used by the community and the surrounding environment are essential media for living Ae. aegypti larvae. This recent study aimed to detect the longevity of Ae. aegypti larvae in different water sources in Surabaya and the killing effect of temephos. An analytical observational and experimental study was conducted in August-September 2021. Twenty-instar III Ae. aegypti larvae were put in each 100 ml beaker glass containing different water sources, such as rain, well, mineral, new and used bath water, and antiseptic soapy water. Fungi in water sources were examined. Two groups were set with and without temephos, the final temephos concentration was of 0.00001 ppm. Live Ae. aegypti larvae, pupae, mosquitoes were observed every 24 hours for seven days without feeding. Living larvae were still found on Day 7 in all water sources with and without temephos. There were more larvae live in soapy water without temephos, particularly on Day 2 to Day 6, compared to other water sources either without or with temephos. In contrast, many larvae died in mineral water with temephos. Some larvae turned into pupae, started on Day 1. Pupae and mosquitoes were mostly found in rain water with temephos. Ae. aegypti larvae survived better in soapy water either with or without temephos. Temephos seemed to be effective to kill Ae. aegypti larvae in mineral water, and might induce larvae in turning to pupae and mosquitoes quickly at low concentration. Keywords: Ae. Aegypti, larvae, water sources, Surabaya ABSTRAK Aedes aegypti menularkan virus dengue penyebab Infeksi Virus Dengue. Penyakit ini terjadi tertinggi di Asia dan menempati urutan pertama setiap tahun, termasuk Surabaya, Indonesia. Faktor penyebab tingginya angka kasus IVD adalah keberadaan tempat perkembangbiakan larva Ae. aegypti. Sumber air yang dimanfaatkan oleh masyarakat dan lingkungan sekitar merupakan media yang penting bagi kehidupan larva Ae. aegypti. Penelitian terbaru ini bertujuan untuk mendeteksi keberlangsungan hidup Ae. aegypti di berbagai sumber air di Surabaya dan efek membunuh temefos. Studi observasional analitik dan eksperimental dilakukan pada bulan Agustus-September 2021. Dua puluh instar III Ae. Larva aegypti dimasukkan ke dalam masing-masing gelas beker 100 ml yang berisi sumber air yang berbeda, seperti air hujan, sumur, mineral, air mandi baru dan bekas, dan air sabun antiseptik 0,5 ppm. Jamur dalam sumber air diperiksa. Dua kelompok ditetapkan dengan dan tanpa temefos, dengan konsentrasi temefos akhir 0,00001 ppm. Larva Ae. aegypti yang hidup, pupa, nyamuk diamati setiap 24 jam selama 7 hari tanpa diberi makan.Banyak Larva yang hidup * Corresponding Author: sukmab@fk.unair.ac.id IJTID, p-ISSN 2085-1103, e-ISSN 2356-0991 Open access under CC-BY-NC-SA Share alike 4.0