_____________________________________________________________________________________________________ ++ Supervisors; *Corresponding author: E-mail: melfordcmiller@gmail.com; Cite as: Melford, Chinwebudu Miller, George G. Simeon, and Martin Mie-Ebi Wankasi. 2024. “Assessment of Biomarkers of Glycation in Type I, Type II and Gestational Diabetes Mellitus”. International Journal of Biochemistry Research & Review 33 (6):544-53. https://doi.org/10.9734/ijbcrr/2024/v33i6935. International Journal of Biochemistry Research & Review Volume 33, Issue 6, Page 544-553, 2024; Article no.IJBCRR.127521 ISSN: 2231-086X, NLM ID: 101654445 Assessment of Biomarkers of Glycation in Type I, Type II and Gestational Diabetes Mellitus Chinwebudu Miller Melford a* , George G. Simeon a++ and Martin Mie-Ebi Wankasi a++ a Department of Chemical Pathology, Faculty of Medical Laboratory Science, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: https://doi.org/10.9734/ijbcrr/2024/v33i6935 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/127521 Received: 08/10/2024 Accepted: 10/12/2024 Published: 14/12/2024 ABSTRACT Diabetes Mellitus, a leading global health challenge, necessitates reliable diagnostic and management biomarkers. This study assessed the efficacy of glycation markers in monitoring glycemic status across Type I, Type II, and gestational diabetes in Port Harcourt, Nigeria. A cross- sectional analysis of 120 participants (40 per diabetes type) evaluated fasting blood glucose (FBG), glycated hemoglobin (HbA1c), glycated albumin (GA), fructosamine (FA), and 1,5-anhydroglucitol (1,5-AG), along with antioxidant markers (glutathione and alpha-tocopherol). Findings indicated that HbA1c remains the most reliable indicator of glycemic status, with a strong correlation to FBG (r² = 0.99). GA and FA demonstrated utility as short-term markers but were less robust than HbA1c. Original Research Article