Research Article CurrentPerspectivesinDetectionofBioactiveCompoundsFrom Costus spicatus Through GC–MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications Dharmar Manimaran, 1 Ararsa Tessema , 2 M. Govindarajulu Yadav, 2 S. Parthasarathi, 3 and Vasan Palanisamy 1 1 Department of Animal Nutrition, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India 2 Department of Food Engineering, Arba Minch University, Arba Minch, Ethiopia 3 Department of Biotechnology, Paavai Engineering College, Namakkal, Tamil Nadu, India CorrespondenceshouldbeaddressedtoArarsaTessema;ararsa251@gmail.com Received4March2024;Revised20August2024;Accepted21December2024 AcademicEditor:SushilNagar Copyright©2025DharmarManimaranetal.BiochemistryResearchInternationalpublishedbyJohnWiley&SonsLtd.Tisisan open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Membersofthe Costus genusaretheconventionalmedicinalplantsusedinthetherapeuticmanagementofnumerousailments, especially for their antioxidant and pharmacological activities. Te crude extract of Costus spicatus was profled using high- resolution GC–MS and LC-MS/MS techniques to determine possible bioactive compounds that are vital to the antioxidant activity. A total of 52 and 63 bioactive compounds have been detected in GC–MS chromatograms using diferent solvents (methanol and ethanol) in C. spicatus leaf extracts, representing the presence of certain bioactive compounds. Te identifed bioactive compounds in both extracts which exhibit neuroprotective efects have been confrmed through various literature studies. Tey are cholestan-3-amine, loliolide, stigmasterol and methylprednisolone acetate, succinimide, fumaric acid, beta- tocopherolandgamma-tocopherol.TeaqueousextractpossessedthehighestantioxidantactivityforDPPHscavengingactivity and lipid peroxidation inhibition assays, whereas the alcoholic aqueous extract showed superior efcacy for hydroxyl radical scavengingactivity.Inthisstudy,weperformedmoleculardockingandfoundthatfourcompoundsexhibitedpromisingbinding afnitieswithpredictedbindingsitesonalpha-synuclein.Notably,Androsta[17-16-b] furan-5′-imine,4′-methylene-3-methoxy- N-cyclohexyl-showedthehighestdockinginteractionscoreof −7.4,indicatingastrongbindingafnity.Tese fndings,combined withthepresenceofbioactivecomponentsinthecrudeextractof C. spicatus,suggestthatthisplantmaypossessneuroprotective properties, warranting further investigation for potential industrial applications in the development of neuroprotective agents. Keywords: antioxidant activities; bioactive compounds; Costus spicatus; GC–MS; LC-MS/MS 1.Introduction Progressivelossorevendeathinthestructureandfunction ofneuronsisthecharacteristicfeatureofneurodegenerative diseases, which has a signifcant adverse efect on both humans and society. Healthcare professionals are still un- certainoftheprecisecausationofcertainneurodegenerative illnesses.Proteindestruction,oxidativestress,infammation, lifestyle exposures, mitochondrial defects, genetic factors and aberrant protein build-up in neurons are a few of the often-investigated environmental root causes of neurode- generative illnesses [1]. Natural supplements are actively employed as a kind of holistic therapy due to their multi- component strategy to target many areas for their mecha- nism of action [2]. Furthermore, their composition in asingledeliverymodeandlackofadverseefectsmakethem potentialcandidatesfortreatingavarietyofcentralnervous system (CNS) disorders [3]. Tese herbal preparations can Wiley Biochemistry Research International Volume 2025, Article ID 9599942, 15 pages https://doi.org/10.1155/bri/9599942