Int.J.Curr.Microbiol.App.Sci (2015) 4(8): 770-777 770 Original Research Article Detection of Antibodies to Mycoplasma mycoides subspecies mycoides in Cattle using Competitive Enzyme-Linked Immunosorbent Assay Jasini A. Musa 1 *, Livinus T. Ikpa 2 , Fati A. Lawan 1 , Enenche F.Ejeh 1 and Quagar, J. Tizhe 3 1 Department of Veterinary Microbiology and Parasitology, University of Maiduguri, Nigeria 2 Bacterial Research Division, National Veterinary Research Institute, Vom, Jos, Nigeria 3 Department of Animal Health and production, College of Agriculture Ganye, Nigeria *Corresponding author ABSTRACT Introduction Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), an insidious, infectious and highly contagious disease of cattle and water buffaloes (Thiaucourt et al., 2013; Amanfu, 2009; Schubert et al., 2011). It is a trans-boundary animal disease that is notifiable to the World Organization of Animal Health- Office International des Epizooties (OIE). Contagious bovine pleuropneumonia has remained a threat to livestock producers and to the well-being of cattle population in Africa due to its prevalence, endemic nature, huge economic losses and social consequences (Egwu et al., 1996; Aliyu et al., 2000; Tambi et al., 2006; Swai et al., 2013). Clinically, CBPP is characterized by anorexia, abduction of the fore-limbs, arched back, distention of the head, ISSN: 2319-7706 Volume 4 Number 8 (2015) pp. 770-777 http://www.ijcmas.com Contagious bovine pleuropneumonia caused by Mycoplasma mycoides subsp. mycoides has remained a threat to livestock producers and to the well-being of cattle population in Africa, where the disease is still prevalent and endemic. Competitive enzyme linked immunosorbent assay (cELISA) is now been recommended as an alternative to complement fixation test (CFT) and for the OIE official method that is applicable for the diagnosis of CBPP. Competitive ELISA is principled on the basis of monoclonal antibody named Mab 117/5. Detection of antibodies to Mmm was carried out according to the standard method using CBPP serum competition ELISA - Version P05410/02 from CIRAD / Institut POURQUIER. Out of the total of 160 sera tested, the Percentage Inhibition (PI) of sera at OD=450nm revealed 1(0.63%). CBPP positive sera for sex was 1.03% in a female animal, 1.32% in one aged >4-6 years and 2.27% for Borno red (Wadara) breed. The overall finding revealed that 0.63% of the detectable antibodies to Mmm was found in a single animal as compared to 159 (99.38%) which tested negative with cELISA. Consequently, our finding has detected the presence of an antibody to Mmm in a serum of cattle at slaughter in Maiduguri abattoir, Northeastern Nigeria. Keywords Antibodies, abattoir, cattle, detection, cELISA, Mycoplasma mycoides subsp. mycoides, Nigeria