ASIAN JOURNAL OF CHEMISTRY ASIAN JOURNAL OF CHEMISTRY https://doi.org/10.14233/ajchem.2017.20472 INTRODUCTION Fish has vital importance as human food source. High quality proteins are obtained from fish, almost 16 % animal proteins are provided by fish consumed by total world ’ s popu- lation. Freshwater fishes are also major source of nutritionally important lipids and fatty acids. Long chain polyunsaturated fatty acids (PUFAs) are also obtained from fish. Polyunsatu- rated fatty acids play an essential role in natural growth, evolu- tion and reproduction of all vertebrates [1]. Aquaculture can produce long-lasting profits for global food security and economic growth, if it is practiced with responsibility [2]. Tilapias are known as the second major group of wild- captured fish, with a commercial production of 2.5 million tons in 2007. Tilapia represented more or less 84 % of total worldwide production of tilapia in 2008 [3]. The expected worldwide production of Nile tilapia will be 2.5 million tons in 2020 [4]. Tilapia provides proteins, phosphorus, potassium, vitamin B-12, niacin and selenium. Its meat has low concen- trations of fats, saturated fats, sodium, carbohydrates, calories and omega-3 fatty acids [5,6]. Fish live as a free organism since evolution of life. It depends on its natural (innate) immune system as compared to higher Estimation of Monthly Variations of Lymphatic Proteins of Tilapia Reared in Pond Water FATIMA JALAL 1,* , MUNIZAH JAVAID 1 , MUHAMMAD IBRAHIM RAJOKA 2 , NUAREEN RANA 3 , GULNAZ AFZAL 4 , SALMA SULTANA 1 and TAYYABA SULTANA 1 1 Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan 2 Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan 3 Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan 4 Department of Zoology, University of Gujrat, Gujrat-50700, Pakistan *Corresponding author: E-mail: fatima_jalal@hotmail.com Received: 26 December 2016; Accepted: 15 March 2017; Published online: 10 April 2017; AJC-18341 The development of functional T- and B-cells from the thymocytes is a crucial step in development of functional immune system and the organs like spleen, kidney and thymus are vital sites to study the lymphatic proteins. Different lymphatic proteins isolated from teleosts but still a little bit known about these proteins. Estimation of proteins from tilapia was done first time in Pakistan. Isolation of lymphatic proteins was performed after tissue rupturing and all the cellular debris was removed. The determined average comparative quantitative estimation of lymphatic proteins concentrations in 6 months tenure in male tilapia's organs was as testes (8.705 mg/mL) > kidney (7.577 mg/mL) > gills (7.556 mg/mL) > spleen (7.455 mg/mL) > alimentary canal (7.243 mg/mL) and in female tilapia as ovaries (9.098 mg/mL) > kidney (7.742 mg/mL) > spleen (7.548 mg/mL) > gills (7.532 mg/mL) > alimentary canal (6.920 mg/mL). High concentration of protein was investigated in reproductive organs (testes, 9.41 mg/mL and ovaries, 9.82 mg/mL) in both sexes. Keywords: Tilapia, Lymph proteins, Organs, PAGE. Asian Journal of Chemistry; Vol. 29, No. 6 (2017), 1291-1295 vertebrates which depend on their acquired immune system [7,8]. Basic defence mechanism in fish is non-specific immu- nity. This nonspecific immunity also significantly affects the acquired immune response. A mechanism of receptor proteins affects homeostasis and acquired immune response. Specific molecular patterns are the distinctive feature of pathogenic microorganisms, viral RNA, bacterial DNA, peptidoglycan, polysaccharides, lipopolysaccharide (LPS) and other molecules. These molecular patterns are recognizable receptor proteins. Immune response is suppressed by various internal and external factors management and density, while efficiency of immune response can be increased by several food additives and immuno- stimulants [9]. Fish do not have bone marrow, even though T cells are generated in thymus [10]. Lymph nodes, peyer patches or germinal centers are absent in fish which describes that antigen production, may be different. Secondary humoral responses in fish are not as elevated as in mammals, although minor immunity maturation [11] and no immunoglobulin class switching is reconized [12]. Lymphoid organs of ample size in teleosts include spleen, thymus, anterior and middle kidneys [13]. The lymphoid bark tissue is covered with a capsule like organ called thymus. It