~ 740 ~ Journal of Pharmacognosy and Phytochemistry 2018; 7(3): 740-747 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(3): 740-747 Received: 01-03-2018 Accepted: 05-04-2018 Dr. Aparna Saraf Associate Professor, The Institute of Science, 15, Madame Cama Road, Fort, Mumbai, Maharashtra, India Pooja Shinde Research Scholar, The Institute of Science, 15, Madame Cama Road, Fort, Mumbai, Maharashtra, India Correspondence Dr. Aparna Saraf Associate Professor, The Institute of Science, 15, Madame Cama Road, Fort, Mumbai, Maharashtra, India Phytochemical analysis and chemical fingerprinting of Derris heyneana (Wight and Arn.) Benth Dr. Aparna Saraf and Pooja Shinde Abstract Origin of the most of the modern day pharmaceuticals can be traced to natural compounds. Herbal pharmaceutical industry suffers from inadequate knowledge of active constituents, their efficacy and dosage. The emerging recognition of herbals and recent development in analytical science, are gradually leading to the development standardized parameters for herbal evaluation. Derris heyneana (Wight and Arn.) Benth. is less known woody climber collected from western ghats region of India. Preliminary phytochemical analysis to detect the presence of various secondary metabolites was performed in six solvents of varying polarity. HPTLC fingerprint and FTIR spectra may be utilized for the purpose of authentication and identification of the crude plant drug by the pharmaceutical industry. Keywords: Derris heyneana (Wight and Arn.) benth. HPTLC fingerprint, FTIR Introduction Various pharmacopoeia and WHO are coming up with global standards for evaluation of quality and standardization parameters for the evaluation of herbals [1-4] . There are different and newer techniques to standardize raw drug and finished products. The standardization of raw herbal drug can be done by using parameters prescribed by various pharmacopoeia and WHO [5] . HPTLC fingerprint is increasingly being utilized for the purpose of quality control, species identification and authentication [6] . It is an important tool to evaluate consistency of crude herbal drugs. HPTLC fingerprint is an accepted parameter in herbal drug assessment protocols in various pharmacopoeias. HPTLC fingerprint of botanically authenticated raw material is used as a primary reference for the purpose of identification and authentication of unknown herbal sample [7-8] . Popularity of HPTLC analytical method for analysis of herbal drugs due to economic, rapid, simultaneously screening of large number of herbal samples and less time consuming methods [9] . FTIR spectroscopy has developed quickly due to its low noise, rapid speed, high repeatability, easy operation, low expense, and so on. FTIR has become increasingly useful in the field of evaluating herbal qualities [10-11] . FTIR spectroscopy has been shown to be a valuable tool for differentiating and classifying closely related species of plants. It is reported that FTIR could be successfully exploited for determining phylogenetic relationships between flowering plants [12] . The different species of Derris has been worked upon and has proven to be good source of secondary metabolites. They have exhibited good pharmacological activities. Literature survey reveals D. heyneana has not been much worked upon for secondary metabolites and pharmacological activities. With this background current work was undertaken with the objective of phytochemical analysis and chemical fingerprinting of Derris heyneana (Wight and Arn.) Benth. Material and Methods The whole plant parts of Derris heyneana (Wight and Arn.) Benth. was collected from Amboli region of Western Ghats of Maharashtra. The herbarium of plant was authenticated by comparing specimen herbarium of ref. no. 15055 at Blatter herbarium, St. Xavier’s college, Mumbai. Plant part was then washed to get rid of dust load and shade dried. It was then grounded using mechanical grinder and fine powder of plant material was used for further studies.