J. Physiol. Biochem., 59 (3), 161-168,2003 Effect of tyramine, a dietary amine, on glycerol and lactate release by isolated adipocytes from old rats C. Bairras, C. Ferrand and C. Atgie Unite de Nutrition et Signalisation Cellulaire (site d'Agen), Universite Bordeaux 1 - DUSA, Avenue Michel Serres, 47000 Agen, France (Received on July 3, 2003) C. BAIRRAS, C. FERRAND and C. ATGIE. Effect of tyramine, a dietary amine, on glycerol and lactate release by isolated adipocytes from old rats. J. Physiol. Biochem., 59 (3),161-168,2003. Amine degradation by adipocyte amine oxidases leads to the production of metabolites that interact with lipid and glucose metabolisms and their hormonal reg- ulations. To further investigate these interactions, we determined the effect of a dietary amine, tyramine (TYR), on glycerol and lactate releases, respectively taken as indices of lipolytic and glycolytic activities of isolated adipocytes. Old male Wistar rats were used to prepare adipocytes by collagenase dissociation of retroperitoneal fat pads. The two tested doses of tyramine (10 pM and 1 mM) had no effect on basal glycerol release. On the other hand, TYR, at the highest dose tested (1 mM), weakly but significantly increased basal lactate release, which was elevated in adipocytes from old rats. Norepinephrine (NE), highly stimulated adipocyte lipolysis with a submaximal effect at 1 pM which was slightly but significantly inhibited by TYR 1 mM. Insulin 1 nM (INS) also poorly inhibited the NE-stimulated lipolysis in adipocytes isolated from old rats. TYR was able to potentiate the poor antilipolytic efficiency of INS. Under similar conditions, a high dose of NE greatly reduced lac- tate production and TYR (1 mM) reversed this inhibition of lactate release. INS was also able to totally reverse the inhibitory effect of NE on lactate release, but there was no potentiation between insulin and tyramine effects. It can be concluded that high doses of TYR interact with norepinephrine and insulin, at least on the control of glycerol and lactate release, by counteracting catecholamine effects and by mimick- ing insulin actions. Key words: Adipose tissuemetabolism, Insulin, Norepinephrine,Glucosemetabolism, Lypolysis, Glycolysis. Correspondenceto C. Atgie (e-mail: c.atgie@-dus-agen.u-bordeaux1.fr).