Research Article Development and Evaluation of Novel Bionanoparticles loaded with Nanosized Lamotrigine by using the Novel Isolated Biopolymer from Phaseolus vulgaris Seeds Sushant Kumar 1* , Nookala Venkala Satheesh Madhav 2 , Anurag Verma 3 , Kamla Pathak 1 1 Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Etawah-206130, Uttar Pradesh, India 2 Faculty of Pharmacy, DIT University, Dehradun-248009, Uttarakhand, India 3 School of Pharmaceutical Sciences, IFTM University, Moradabad-244001, Uttar Pradesh, India Introduction The word biopolymer has drawn the attention of researchers as a novel biomaterial for development and designing of targeted drug delivery systems. [1] Nowadays, the drawbacks associated with synthetic and semi- synthetic polymers are very challenging problems in drug delivery system designing. The synthetic and semi- synthetic polymers are the first choice in development of targeted drug delivery systems. But these polymers have a number of side effects which may produce different patient incompatibility. Article history: Received: 14 February, 2020 Revised: 11 April, 2020 Accepted: 30 April, 2020 Published: 30 May, 2020 Keywords: Bionanosuspension, Biopolymer, Lamotrigine, Phaseolus vulgaris. DOI: 10.25004/IJPSDR.2020.120306 The aim of this research was to isolate and characterize the novel biopolymer from Phaseolus vulgaris seeds and to formulate lamotrigine loaded bionanoparticles using this biomaterial. The biopolymer was isolated by simplified and economical process and analyzed for different physicochemical and spectral properties. The nanosizing of lamotrigine was done by bath sonication method, and its particle size in nano-range was screened by UV method. The bionanosuspension loaded with nanosized lamotrigine was prepared by bath sonication for 15 cycles (1 cycle equal to 3 minutes). The formulated bionanosuspension loaded with lamotrigine using biopolymer was compared with lamotrigine loaded bionanosuspension by using hydroxypropyl methyl cellulose (HPMC). The bionanosuspension was subjected for pH study, dispersibility study, % entrapment efficiency, nanosizing screening, particle size study, and in vitro study for drug release and stability study of formulated bionanosuspension. The best formulation LPVu7 showed up to 90.36 ± 0.9% drug release in 36 hours having t 50% of 17 hours and t 80% of 29 hours with r 2 value of 0.9927 as compared to release from LSP10 with 92.43 ± 0.68%, having t 50% of 16 hours and t 80% of 28 hours with r 2 value of 0.9951 in 36 hours. The results reveal that isolated biopolymer may be used as an alternative to synthetic polymer because of its novel inbuilt bioretardant cum biostabilizing properties. The formulated bionanosuspension is feasible for delivering the nanosized lamotrigine in sustained manner for prolonged time for long term treatment of epilepsy. Contents lists available at UGC-CARE International Journal of Pharmaceutical Sciences and Drug Research [ISSN: 0975-248X; CODEN (USA): IJPSPP] journal home page : http://ijpsdr.com/index.php/ijpsdr International Journal of Pharmaceutical Sciences and Drug Research 2020; 12(3): 247-254 * Corresponding Author: Mr. Sushant Kumar Address: Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Etawah-206130, Uttar Pradesh, India Email : k.sushant25@gmail.com Relevant conflicts of interest/financial disclosures: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Copyright © 2020 Sushant Kumar et al. This is an open access article distributed under the terms of the Creative Commons Attribution- NonCommercial- ShareAlike 4.0 International License which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. ABSTRACT ARTICLE INFO The synthetic and semi-synthetic polymers may produce a number of long term toxic effects. The polymers may produce localized side effects, acute and chronic unwanted effects, like skin and eye irritation with methyl methacrylate and poly methyl methacrylate (PMMA). Administration of povidone on intramuscular administration may accumulate in organs and may cause toxic effects. Carbomer dust may cause eye irritation and respiratory problems. These polymers on acid degradation, the degradation product may be polyglycolide, polylactide, and copolymers may cause the local reaction from the acid